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| Content Provider | Springer Nature Link |
|---|---|
| Author | Cecilia, D. Kakade, M. Alagarasu, K. Patil, J. Salunke, A. Parashar, D. Shah, P. S. |
| Copyright Year | 2014 |
| Abstract | Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously detect and quantitate RNA of all dengue virus (DENV) serotypes and chikungunya virus (CHIKV). The sensitivity was 100 % for DENV and 95.8 % for CHIKV, whilst the specificity was 100 % for both viruses when compared with conventional RT-PCR. The detection limit ranged from 1 to 50 plaque-forming units. The assay was successfully used for differential diagnosis of dengue and chikungunya in Pune, where the viruses co-circulate. |
| Starting Page | 323 |
| Ending Page | 327 |
| Page Count | 5 |
| File Format | |
| ISSN | 03048608 |
| Journal | Archives of Virology |
| Volume Number | 160 |
| Issue Number | 1 |
| e-ISSN | 14328798 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2014-09-19 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dengue chikungunya multiplex real time RT-PCR assay diagnosis Virology Medical Microbiology Infectious Diseases |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Medicine |
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