NDLI: Dual-layered and double-targeted nanogold based lateral flow immunoassay for influenza virus

Content Provider	Springer Nature Link
Author	Wiriyachaiporn, Natpapas Maneeprakorn, Weerakanya Apiwat, Chayachon Dharakul, Tararaj
Copyright Year	2014
Abstract	We report on a highly sensitive lateral flow immunoassay (LFIA) for influenza A which serves as a model antigen. Gold nanoparticles conjugated to monoclonal antibodies specific to the two most abundant influenza A proteins, nucleoprotein and matrix protein, were used as detector probes. Using this approach, the nucleoprotein and matrix protein in the virion were detected simultaneously. The signal was further amplified via a signal amplification strategy that is making use of two-layered nanogold in combination with a double-targeted detection format. Under optimized conditions, the system is capable of detecting influenza A antigens in infected cells at levels as low as 47 TCID50 · mL−1 (50 % tissue culture infectious dose) within 15 min. Compared to the conventional LFIA based on single-targeted detection, the detection capability of this system is better by a factor of 8 without requiring additional steps or instruments. In addition to its simplicity and rapidity, this LFIA also can detect the target analyte in even complex biological matrix. This proof-of-principle of a dual-layered and double-targeted nanogold-based LFIA is deemed to be useful for developing single-step, rapid, and sensitive tests for screening and diagnosis. Figure Dual-layered and double-targeted nanogold based lateral flow immunoassay was developed for a sensitive detection of influenza. The enhanced sensitivity was achieved via the increased number of the target binding sites and the use of the signal-amplifying probes without additional steps
Starting Page	85
Ending Page	93
Page Count	9
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	182
Issue Number	1-2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-06-07
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dual-layered and double-targeted nanogold Lateral flow immunoassay Double-targeted detection Influenza A antigen Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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