NDLI: Flow based immuno/bioassay and trends in micro-immuno/biosensors

Content Provider	Springer Nature Link
Author	Hartwell, Supaporn Kradtap Grudpan, Kate
Copyright Year	2010
Abstract	The term immuno/bioassay refers to analytical techniques that utilize the specific molecular recognition between antibodies and antigens or between biomolecules and specific receptors. Features such as high selectivity and low sample consumption make them very useful for analysis of samples with complex matrices. However, immuno/bioassays involve time-consuming (multi-step) operations which usually consist of steps of multiple incubation and washing. These are tedious and may result in large errors. Automatted immuno/bioassay systems can ease and shorten these processes and thus are highly beneficial. The hyphenation of flow-based techniques (i.e. flow injection related techniques and micro-fluidic systems) with immuno/bioassay protocols paves a new way for performing such assays. Compared to conventional micro-plate formats, flow-based immuno/bioassays can reduce the time needed for analysis, the volumes of samples and reagents consumed, and the need for trained personnel. In order to transform immuno/bioassays from conventional to flow-based formats, the solid surfaces used for the immobilization step has to be changed in order to meet the specific requirements of flow systems. To further develop the on-site analytical systems in micro-fluidic platforms, improvements in detection methods are necessary for high-sensitivity and rapid measurement. This review overviews the advantages and disadvantages of flow-based immuno/bioassay formats, the various types of solid surfaces for immobilization, and the methods of detection. Trends to improve sensitivity, speed and robustness are emphasized.
Starting Page	201
Ending Page	220
Page Count	20
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	169
Issue Number	3-4
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2010-03-29
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flow injection Immunoassay Bioassay Sensors Microengineering Analytical Chemistry Characterization and Evaluation of Materials Nanotechnology Nanochemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Conducting polymer composites with graphene for use in chemical sensors and biosensors

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

A real-time immuno-PCR method for detecting 3,3′,4,4′-tetrachlorobiphenyl

Electrochemical sensors based on graphene materials

Carbon nanotubes and graphene in analytical sciences

Nanomaterials-based electrochemical sensors for nitric oxide

Highly sensitive electrochemical immunoassay for zearalenone in grain and grain-based food

DNAzyme based electrochemical sensors for trace uranium

Electrochemical lectin based biosensors as a label-free tool in glycomics

Flow based immuno/bioassay and trends in micro-immuno/biosensors

Similar Documents

Conducting polymer composites with graphene for use in chemical sensors and biosensors

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

A real-time immuno-PCR method for detecting 3,3′,4,4′-tetrachlorobiphenyl

Electrochemical sensors based on graphene materials

Carbon nanotubes and graphene in analytical sciences

Nanomaterials-based electrochemical sensors for nitric oxide

Highly sensitive electrochemical immunoassay for zearalenone in grain and grain-based food

DNAzyme based electrochemical sensors for trace uranium

Electrochemical lectin based biosensors as a label-free tool in glycomics

Flow based immuno/bioassay and trends in micro-immuno/biosensors