NDLI: Hollow nanogold microsphere-signalized lateral flow immunodipstick for the sensitive determination of the neurotoxin brevetoxin B

Content Provider	Springer Nature Link
Author	Zhang, Kangyao Wu, Jiachang Li, Yineng Wu, Yarong Huang, Tianfu Tang, Dianping
Copyright Year	2014
Abstract	We report on a new membrane-based lateral-flow assay for the rapid determination of the algal biotoxin brevetoxin B (BrevTox) that can be found in fish. Hollow nanogold microspheres (HGMS) with an average size of 45 nm were synthesized by using the reverse micelle method, followed by functionalization with monoclonal mouse anti-BrevTox antibody (mAb-HGMS). They served as the signalling reagent. Bovine serum albumin-BrevTox conjugate and rabbit anti-mouse IgG antibody were manually spotted on the nitrocellulose membrane and served as reagents for the test line and control line, respectively. Both spherical gold nanoparticle-labeled anti-BrevTox antibody (mAb-AuNP) and mAb-HGMS conjugate were investigated with respect to their suitability as signal-transduction tags, and the latter was found to give improved analytical features. The HGMS-based labeling method was then also studied in terms of performance in the immunodipstick assay. Under optimal conditions, the visual detection limit (cut-off value) of the mAb-AuNP based assay is 1.5 ng mL−1, while the sensitivity of the mAb-HGMS based assay is as low as 0.1 ng mL−1. Yes/no decisions can be made within 10 min without the need for expensive instrumentation. The results for the analysis of target BrevTox in spiked fish samples showed a good correlation with data obtained with the commercial ELISA. Importantly, the assay gave no false negative results. Figure We report on a new membrane-based lateral-flow assay for the rapid determination of the algal biotoxin brevetoxin B that can be found in fish. These results showed a good correlation with data obtained with the commercial ELISA. Importantly, the assay gave no false negative results.
Starting Page	1447
Ending Page	1454
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	11-12
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-05-28
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hollow nanogold microsphere Brevetoxin B Lateral-flow immunodipstick Biotoxin Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Lateral flow immunodipstick for visual detection of aflatoxin B1 in food using immuno-nanoparticles composed of a silver core and a gold shell

Dual-layered and double-targeted nanogold based lateral flow immunoassay for influenza virus

A highly sensitive method for determination of paracetamol by adsorptive stripping voltammetry using a carbon paste electrode modified with nanogold and glutamic acid

Dopamine sensor based on a hybrid material composed of cuprous oxide hollow microspheres and carbon black

Integration of lateral flow and microarray technologies for multiplex immunoassay: application to the determination of drugs of abuse

Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles

Immunochromatographic lateral flow strip for on-site detection of bisphenol A

Glucometer-based quantitative determination of Hg(II) using gold particle encapsulated invertase and strong thymine-Hg(II)-thymine interaction for signal amplification

Sensitive detection of hydrogen peroxide in foodstuff using an organic–inorganic hybrid multilayer-functionalized graphene biosensing platform

Hollow nanogold microsphere-signalized lateral flow immunodipstick for the sensitive determination of the neurotoxin brevetoxin B

Similar Documents

Lateral flow immunodipstick for visual detection of aflatoxin B1 in food using immuno-nanoparticles composed of a silver core and a gold shell

Dual-layered and double-targeted nanogold based lateral flow immunoassay for influenza virus

A highly sensitive method for determination of paracetamol by adsorptive stripping voltammetry using a carbon paste electrode modified with nanogold and glutamic acid

Dopamine sensor based on a hybrid material composed of cuprous oxide hollow microspheres and carbon black

Integration of lateral flow and microarray technologies for multiplex immunoassay: application to the determination of drugs of abuse

Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles

Immunochromatographic lateral flow strip for on-site detection of bisphenol A

Glucometer-based quantitative determination of Hg(II) using gold particle encapsulated invertase and strong thymine-Hg(II)-thymine interaction for signal amplification

Sensitive detection of hydrogen peroxide in foodstuff using an organic–inorganic hybrid multilayer-functionalized graphene biosensing platform

Hollow nanogold microsphere-signalized lateral flow immunodipstick for the sensitive determination of the neurotoxin brevetoxin B