NDLI: Titanium dioxide nanorod-based amperometric sensor for highly sensitive enzymatic detection of hydrogen peroxide

Content Provider	Springer Nature Link
Author	Li, Qian Cheng, Kui Weng, Wenjian Du, Piyi Han, Gaorong
Copyright Year	2013
Abstract	Titanium dioxide nanorods (TNR) were grown on a titanium electrode by a hydrothermal route and further employed as a supporting matrix for the immobilization of nafion-coated horseradish peroxidase (HRP). The strong electrostatic interaction between HRP and TNR favors the adsorption of HRP and facilitates direct electron transfer on the electrode. The electrocatalytic activity towards hydrogen peroxide (H2O2) was investigated via cyclic voltammetry and amperometry. The biosensor exhibits fast response, a high sensitivity (416.9 μA·mM−1), a wide linear response range (2.5 nM to 0.46 mM), a detection limit as low as 12 nM, and a small apparent Michaelis-Menten constant (33.6 μM). The results indicate that this method is a promising technique for enzyme immobilization and for the fabrication of electrochemical biosensors. Figure A TiO2 nanorod film was directly grown on Ti substrate by a hydrothermal route, and was further employed for a supporting matrix to immobilize horseradish peroxidase as a biosensor electrode. The as-prepared hydrogen peroxide biosensor based on Nafion/HRP/TNR/Ti electrode exhibited fast response and excellent electrocatalytic activity toward H2O2, i.e., a high sensitivity (416.9 μA mM−1), a wide linear range (2.5 × 10−8 to 4.6 × 10−4 M) with a low detection limit (0.012 μM) and a small apparent Michaelis-Menten constant (33.6 μM).
Starting Page	1487
Ending Page	1493
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	180
Issue Number	15-16
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-10-03
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	TiO2 nanorod film Hydrogen peroxide biosensor Horseradish peroxidase Electrocatalysis Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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