NDLI: Surface molecularly imprinted polydopamine films for recognition of immunoglobulin G

Content Provider	Springer Nature Link
Author	Tretjakov, Aleksei Syritski, Vitali Reut, Jekaterina Boroznjak, Roman Volobujeva, Olga Öpik, Andres
Copyright Year	2013
Abstract	We have prepared a surface imprinted polymer (SIP) film for label-free recognition of immunoglobulin G (IgG). The IgG-SIPs were obtained by covalent immobilization of IgG via a cleavable covalent bond and a suitable spacer unit to a gold electrode, followed by electrodepostion of a nm-thin film of polydopamine (PDA). The IgG was then removed by destruction of the cleavable bond so that complementary binding sites were created on the surface of the film. IgG-SIPs with various thicknesses of the PDA films were compared with respect to their affinity to IgG using a quartz crystal microbalance combined with flow injection analysis. The films were also characterized by cyclic voltammetry and scanning electron microscopy. The IgG-SIPs with a film thickness of around 17 nm showed the most pronounced imprinting effect (IF 1.66) and a binding constant of 296 nM. Figure A strategy for preparation of the IgG-Surface Imprinted Polymeric (IgG-SIP) thin films was developed. IgG was covalently immobilized via a cleavable cross-linker to a gold electrode surface followed by electrochemical deposition of a nanometer thin PDA film. After cleaving S-S bond in the linker the IgG was removed leaving behind the complementary binding sites confined in the surface of the polymer film. The prepared IgG-SIPs were applied for IgG recognition.
Starting Page	1433
Ending Page	1442
Page Count	10
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	180
Issue Number	15-16
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-07-14
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Molecularly imprinted polymers IgG Polydopamine EQCM QCM FIA Electrochemical polymerization Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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