NDLI: Colorimetric and visual determination of DNase I activity using gold nanoparticles as an indicator

Content Provider	Springer Nature Link
Author	He, Yue Cheng, Fen Pang, Dai Wen Tang, Hong Wu
Copyright Year	2016
Abstract	Patients with prostate cancer and systemic lupus erythematosus exhibit reduced DNase I activity, and patients with myocardial infarction exhibit increased DNase I activity. So the assay of DNase I is of high importance. A colorimetric assay is described here for the determination of the activity of DNase. It is based on strand scission of dsDNA as catalyzed by DNase I. The products of digestion (nucleoside monophosphates) can better stabilize citrate capped AuNPs than dsDNA. In the absence of DNase I, the AuNPs aggregate in presence of NaCl and then display a blue color. In the presence of the analyte (DNase I), AuNPs do not aggregate but rather remain dispersed and display a red color. These findings were exploited to design a DNase I activity assay that is based on the measurement of ratio of absorbances at 520 nm (red) to 650 nm (blue). The detection limit for DNase I activity is found to be 7.1 U⋅L−1. In our perception, this assay has a large potential with respect to diagnoses of DNase I activity-related diseases and in drug screening. Graphical Abstract A method is described for the determination of the activity of DNase I. It is based on the capability of nucleoside monophosphates (dNMPs; formed by DNase-catalyzed scission of dsDNA) to stabilize red gold NPs against NaCl-induced aggregation. AuNPs stabilized with dsDNA, in contrast, readily aggregate in presence of NaCl to form blue clusters.
Starting Page	101
Ending Page	106
Page Count	6
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	184
Issue Number	1
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-11-04
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Deoxyribonuclease Enzyme activity assay Visual test Thrombin Cytochrome C Double-stranded DNA Nucleoside monophosphates Drug screening Systemic lupus erythematosus Myocardial infarction Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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