NDLI: Fluorescence correlation spectroscopy of gold nanoparticles, and its application to an aptamer-based homogeneous thrombin assay

Content Provider	Springer Nature Link
Author	Xu, Zhancheng Huang, Xiangyi Dong, Chaoqing Ren, Jicun
Copyright Year	2013
Abstract	We have studied the fluorescence properties and diffusion behaviors of gold nanoparticles (GNPs) in solution by using fluorescence correlation spectroscopy (FCS) at single molecule level. The GNPs display a high photo-saturation feature. Under illumination with strong laser light, they display higher brightness per particle (BPP) despite their low quantum yields. Based on the unique fluorescence properties and diffusion behaviors of GNPs, we have developed a sensitive and homogenous thrombin assay. It is based on a sandwich strategy and is making use of GNPs to which two different aptamers are conjugated. When the differently aptamer-labeled GNPs are mixed with solutions containing thrombin, the affinity reaction causes the GNPs to form dimers or oligomers. This leads to an increase in the diffusion time of the GNPs in the detection volume that is seen in FCS. The FCS method enables sensitive detection of the change in the characteristic diffusion time of the GNPs before and after the affinity reaction. Quantitative analysis of thrombin is based on the measurement of the change in the diffusion time. Under optimal conditions, the calibration plot is linear in the 0.5 nM to 110 nM thrombin concentration range, and the detection limit is 0.5 nM. The method was successfully applied to the direct determination of thrombin in human plasma. Figure On the basis of fluorescence correlation spectroscopy and recognition of aptamers, a new, sensitive and homogenous method for determination of thrombin in human plasma was developed using gold nanoparticles.
Starting Page	723
Ending Page	730
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	7-8
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-11-28
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gold nanoparticles Fluorescent correlation spectroscopy Brightness per particle Thrombin Aptamers Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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