NDLI: Silver nanoparticles modified with thiomalic acid as a colorimetric probe for determination of cystamine

Content Provider	Springer Nature Link
Author	Mohammadi, Somayeh Khayatian, Gholamreza
Copyright Year	2016
Abstract	The authors describe a colorimetric method for highly selective determination of cystamine using silver nanoparticles capped with thiomalic acid (TMA-AgNPs). The TMA-AgNPs exhibit a strong surface plasmon resonance absorption band at 395 nm. After the addition of cystamine to their yellow colloidal solution, the absorption maximum at 395 nm decreases gradually, and a new absorption peak appears at around 560 nm. This is accompanied by a color change, first to green and then to purple. The aggregation of NPs induced by cystamine is assumed to be due to the electrostatic interaction between capped NPs and cystamine. Aggregation was confirmed by UV–vis absorptiometry and transmission electron microscopy. A linear correlation was obtained between the ratio of absorbances at 560 and 395 nm and the cystamine concentration in the range between 0.2 μM and 10.0 μM. The limit of detection is 46 nM. The method was successfully applied to the determination of cystamine in spiked human serum and urine samples. Recoveries ranged between 94.0 % and 100.9 %, and relative standard deviations are <9.50 % (for n = 3). Graphical abstract A rapid and selective colorimetric method for detection of cystamine is described. The electrostatic interactions between positively charged cystamine-capped silver nanoparticles (AgNPs) and negatively charged thiomalic acid capped AgNPs leads to nanoparticle aggregation and a color change from yellow to green.
Starting Page	253
Ending Page	259
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	184
Issue Number	1
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-11-15
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aggregation Surface thiol chemistry Electrostatic interaction Surface plasmonic absorption Serum analysis Urine analysis Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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