NDLI: Direct isolation of differentially expressed genes from a specific chromosome region of common wheat: application of the amplified fragment length polymorphism-based mRNA fingerprinting (AMF) method in combination with a deletion line of wheat

Please wait, while we are loading the content...

Direct isolation of differentially expressed genes from a specific chromosome region of common wheat: application of the amplified fragment length polymorphism-based mRNA fingerprinting (AMF) method in combination with a deletion line of wheat

Extra-ribosomal function(s) of the plastid ribosomal protein L4 in the expression of ribosomal components in spinach

Transformation of Arabidopsis with a Brassica SLG/SRK region and ARC1 gene is not sufficient to transfer the self-incompatibility phenotype

A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp. PCC6301: a soluble 33-kDa polypeptide with high sequence similarity to ribosomal protein S1

Cloning and expression of the S-adenosylmethionine decarboxylase gene of Neurospora crassa and processing of its product

RFLP-based analysis of three RbcS subfamilies in diploid and polyploid species of wheat

A high-resolution linkage map of the vicinity of the rice submergence tolerance locus Sub1

The Drosophila dominant wing mutation Dichaete results from ectopic expression of a Sox-domain gene

Tagging of genes involved in multidrug resistance in Aspergillus nidulans

Differential complementation of a Neurospora crassa Gαi mutation using mammalian Gα protein genes

Homologous recombination and transposition generate chromosome I neopolymorphism during meiosis in Saccharomyces cerevisiae

Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5′ untranslated region followed by gene duplication

Molecular and General Genetics MGG : Volume 263, Issue 3, April 2000

Molecular and General Genetics MGG : Volume 263, Issue 2, March 2000

Molecular and General Genetics MGG : Volume 263, Issue 1, February 2000

Molecular and General Genetics MGG : Volume 262

Molecular and General Genetics MGG : Volume 261

Molecular and General Genetics MGG : Volume 260

Molecular and General Genetics MGG : Volume 259

Molecular and General Genetics MGG : Volume 258

Molecular and General Genetics MGG : Volume 257

Molecular and General Genetics MGG : Volume 256

Molecular and General Genetics MGG : Volume 255

Molecular and General Genetics MGG : Volume 254

Molecular and General Genetics MGG : Volume 253

Direct isolation of differentially expressed genes from a specific chromosome region of common wheat: application of the amplified fragment length polymorphism-based mRNA fingerprinting (AMF) method in combination with a deletion line of wheat

Content Provider	Springer Nature Link
Author	Kojima, T. Habu, Y. Iida, S. Ogihara, Y.
Copyright Year	2000
Abstract	The amplified restriction fragment length polymorphism (AFLP)-based mRNA fingerprinting (AMF) method makes it possible systematically and conveniently to identify differentially expressed cDNAs with high reproducibility. We have applied the AMF method to the cloning of the Q gene of common wheat, which is located on the long arm of chromosome 5A and pleiotropically controls the spike morphology and the threshing character of seeds. Using the AMF method, we compared the fingerprints of mRNA samples extracted from the young spikes of Triticum aestivum cv. Chinese Spring (CS) carrying the Q gene to those of a chromosome deletion line of CS, namely, q5, which lacks 15% of 5AL including the Q gene. Approximately 12,200 fragments were produced after PCR with 256 primer combinations. Of these, 92 fragments were differentially expressed between CS and q5. Northern and Southern analyses showed that 16 fragments gave specific or relatively stronger transcript signals in CS, and these clones were present in single copy or in low copy numbers in the wheat genome. Four clones were genetically mapped to the region deleted in q5. Subsequently, one clone, pTaQ22, was mapped at the same locus as the Q gene, indicating that pTaQ22 corresponds to the Q gene or is tightly linked to it. DNA sequence data showed that pTaQ22 had no homology to any known genes, thus suggesting a novel function for this gene in flower morphogenesis. This AMF method might provide a straightforward method for isolating genes in the hexaploid background of common wheat.
Starting Page	635
Ending Page	641
Page Count	7
File Format	PDF
ISSN	00268925
Journal	Molecular and General Genetics MGG
Volume Number	263
Issue Number	4
e-ISSN	14321874
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-05-16
Publisher Place	Berlin/Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

AFLP: Amplified Fragment Length Polymorphism

Identification of Differentially Expressed Genes by cDNA-Amplified Fragment Length Polymorphism in the Biocontrol Agent Pichia anomala (Strain Kh5).

Evaluation of pcr-restriction profile analysis and is2404 restriction fragment length polymorphism and amplified fragment length polymorphism fingerprinting for identification and typing of (2001).

Rediscovery of historical Vitis vinifera varieties from the South Anatolia region by using amplified fragment length polymorphism and simple sequence repeat DNA fingerprinting methods.

ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping.

Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism

Clonal structure in a dwarf bamboo (Sasa senanensis) population inferred from amplified fragment length polymorphism (AFLP) fingerprints.

Identification of Differentially Expressed Genes by cDNA-Amplified Fragment Length Polymorphism in the Biocontrol Agent Pichia anomala (Strain Kh5).

Identification ofporphyra lines (rhodophyta) by AFLP DNA fingerprinting and molecular markers

Direct isolation of differentially expressed genes from a specific chromosome region of common wheat: application of the amplified fragment length polymorphism-based mRNA fingerprinting (AMF) method in combination with a deletion line of wheat

Similar Documents

AFLP: Amplified Fragment Length Polymorphism

Identification of Differentially Expressed Genes by cDNA-Amplified Fragment Length Polymorphism in the Biocontrol Agent Pichia anomala (Strain Kh5).

Evaluation of pcr-restriction profile analysis and is2404 restriction fragment length polymorphism and amplified fragment length polymorphism fingerprinting for identification and typing of (2001).

Rediscovery of historical Vitis vinifera varieties from the South Anatolia region by using amplified fragment length polymorphism and simple sequence repeat DNA fingerprinting methods.

ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping.

Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism

Clonal structure in a dwarf bamboo (Sasa senanensis) population inferred from amplified fragment length polymorphism (AFLP) fingerprints.

Identification of Differentially Expressed Genes by cDNA-Amplified Fragment Length Polymorphism in the Biocontrol Agent Pichia anomala (Strain Kh5).

Identification ofporphyra lines (rhodophyta) by AFLP DNA fingerprinting and molecular markers

Direct isolation of differentially expressed genes from a specific chromosome region of common wheat: application of the amplified fragment length polymorphism-based mRNA fingerprinting (AMF) method in combination with a deletion line of wheat