NDLI: Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5′ untranslated region followed by gene duplication

Content Provider	Springer Nature Link
Author	Kubo, N. Jordana, X. Ozawa, K. Zanlungo, S. Harada, K. Sasaki, T. Kadowaki, K.
Copyright Year	2000
Abstract	Mitochondrial ribosomal protein S10 (rps10) is encoded by the mitochondrial genome in potato and pea. Here we show that the rps10 gene is absent from the mitochondrial genome of rice and has been transferred to the nucleus. Cloning and transcriptional analysis show that there are two rps10 genes in the rice nuclear genome and that their transcripts differ in abundance. Western analysis detected the RPS10 protein in the soluble fraction of rice mitochondria, although neither RPS10 has any obvious N-terminal presequence for targeting to mitochondria. This result suggests that targeting information is present in the internal region of rice RPS10. Genomic sequence analysis indicated that each rps10 gene has an intron in the 5′ untranslated region (5′ UTR) and that these intron sequences are homologous to each other. This result strongly suggests that a duplication event occurred after transfer of the rps10 gene to the nucleus. The duplicated rps10 genes have since been translocated to different chromosomes, because the two rps10 genes were mapped on chromosomes 6 and 12 by RFLP analysis. Interestingly, the 5′ UTR and the intron of the rice rps10 genes are homologous to sequences found in several rice genes with various functions, such as osk4, EF-1β2 and RAG1, suggesting a common origin and a functional role for the 5′ UTR. Acquisition of the 5′ flanking region might have accelerated the activation of the mitochondrial rps10 gene which was transferred to the nuclear genome.
Starting Page	733
Ending Page	739
Page Count	7
File Format	PDF
ISSN	00268925
Journal	Molecular and General Genetics MGG
Volume Number	263
Issue Number	4
e-ISSN	14321874
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-05-16
Publisher Place	Berlin/Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Genetics

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