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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Massart, Sébastien Jijakli, M. Haissam |
| Description | Country affiliation: Belgium Author Affiliation: Massart S ( Plant Pathology Unit, FacultéUniversitaire des Sciences Agronomiques de Gembloux, Belgium.) |
| Abstract | ABSTRACT cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was used to identify genes potentially involved in biological control, by strain Kh5 (Pichia anomala), of Botrytis cinerea, an important post-harvest pathogen on apples. Strain Kh5 was grown in yeast nitrogen base (YNB) plus glucose (G medium) or YNB plus cell walls of B. cinerea (B medium). Thirty-five primer pairs were used in AFLP amplifications, resulting in a total of more than 2,450 bands derived from the mRNA of strain Kh5 grown in B medium. Eighty-six bands (3.5%) corresponded to genes upregulated in B medium compared with G medium. Of these 86 bands, 28 were selected, cloned, sequenced, and subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR) to confirm their differential expression. An appropriate housekeeping gene, G2, was selected and used to normalize the results of RT-PCR. Eleven genes presented an increased gene expression in the presence of B. cinerea cell walls (expression >1). Statistical analysis showed a significant increase for 5 of these 11 genes. The overexpressed genes show homologies to yeast genes with various functions, including beta-glucosidase, transmembrane transport, citrate synthase, and external amino acid sensing and transport. Some of these functions could be related to cell wall metabolism and potentially involved in mycoparasitic properties. |
| File Format | HTM / HTML |
| ISSN | 0031949X |
| e-ISSN | 19437684 |
| Journal | Phytopathology |
| Issue Number | 1 |
| Volume Number | 96 |
| Language | English |
| Publisher | The American Phytopathological Society |
| Publisher Date | 2006-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Plant Disease Plant Pathology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Agronomy and Crop Science |
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