NDLI: Disruption of the petB-petD intergenic region in tobacco chloroplasts affects petD RNA accumulation and translation

Content Provider	Springer Nature Link
Author	Monde, R. A. Greene, J. C. Stern, D. B.
Copyright Year	2000
Abstract	Translation initiation in chloroplasts is a complex process involving a variety of cis-elements and trans-acting factors. Many chloroplast mRNAs are processed products of polycistronic primary transcripts, but the functional requirement for processing is mostly enigmatic. In tobacco, the petB and petD genes, which encode subunits of the cytochrome b 6 /f complex, are transcribed from the psbB operon, whose primary transcript is processed into products including di- or tricistronic, but not monocistronic, petB and petD mRNAs. To begin to identify elements important for petB and/or petD translation, we generated tobacco chloroplast transformants by inserting selectable aadA marker cassette in the petB-petD intergenic region. The resulting plants required sucrose for growth, and their phenotypes depended on the orientation of the aadA cassette. When aadA was inserted in the same transcriptional orientation as the psbB operon, petB and petD mRNAs were abundantly produced but aberrant in size, and only 25% of the wild-type amount of the cytochrome b 6 /f complex accumulated. With the aadA cassette in the opposing orientation, however, very little petD mRNA accumulated, and the cytochrome b 6 /f complex was undetectable. Polysome analysis suggested that petD mRNAs in both transformants were poorly translated, indicating that the intergenic region contains essential translational elements.
Starting Page	610
Ending Page	618
Page Count	9
File Format	PDF
ISSN	00268925
Journal	Molecular and General Genetics MGG
Volume Number	263
Issue Number	4
e-ISSN	14321874
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-05-16
Publisher Place	Berlin/Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Genetics

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HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs.

Nucleotide sequence of the 5.2 kbp barley chloroplast DNA fragment, containing psbB-psbH-petB-petD gene cluster.

The sequence and secondary structure of the 3′-UTR affect 3′-end maturation, RNA accumulation, and translation in tobacco chloroplasts

HCF152, an Arabidopsis RNA Binding Pentatricopeptide Repeat Protein Involved in the Processing of Chloroplast psbB-psbT-psbH-petB-petD RNAs

The 3 untranslated region of satellite tobacco necrosis virus rna stimulates translation in (1993).

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Function of the Chlamydomonas reinhardtii petD 5' untranslated region in regulating the accumulation of subunit IV of the cytochrome b6/f complex

Disruption of the petB-petD intergenic region in tobacco chloroplasts affects petD RNA accumulation and translation

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