NDLI: Identification and expression analysis of the zebrafish orthologue of Klotho

Content Provider	Springer Nature Link
Author	Suga, Yuya Lardelli, Michael
Copyright Year	2011
Abstract	Klotho is an aging suppressor gene. In mice, loss of Klotho function causes accelerated aging while increased Klotho expression increases longevity. This study aimed to identify and characterize the orthologue of Klotho in zebrafish, a powerful model organism for the investigation of development and human disease. Zebrafish klotho was identified by a bioinformatics approach, and cloning and sequencing of klotho cDNA confirmed the in silico analysis. The zebrafish Klotho protein has a structure similar to human and mouse Klotho, but it lacks an apparent secretory signal sequence. We can find no evidence of an alternative transcript isoform lacking the transmembrane domain coding sequence as seen in mammals. RT-PCR revealed the expression of klotho during embryonic development and in a wider variety of adult tissues than in mouse. Quantitative real-time RT-PCR demonstrated the relative gene expression profile of zebrafish Klotho during embryogenesis and in adult tissues. In situ hybridization showed an apparently diffuse signal of klotho mRNA expression in the adult zebrafish testis.
Starting Page	179
Ending Page	186
Page Count	8
File Format	PDF
ISSN	0949944X
Journal	Development Genes and Evolution
Volume Number	221
Issue Number	3
e-ISSN	1432041X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Klotho Zebrafish RT-PCR Quantitative real-time PCR In situ hybridization Biochemistry Cell Biology Animal Genetics and Genomics Developmental Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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