NDLI: HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development

Content Provider	Springer Nature Link
Author	Sakuma, Tetsushi Ohnishi, Kazuya Fujita, Kazumasa Ochiai, Hiroshi Sakamoto, Naoaki Yamamoto, Takashi
Copyright Year	2011
Abstract	Sulfatases such as arylsulfatase and heparan sulfate 6-O-endosulfatase play important roles in morphogenesis during sea urchin development. For the activation of these sulfatases, Cα-formylglycine formation by sulfatase modifying factor (Sumf) is required. In this study, to clarify the regulatory mechanisms for the activation of sulfatases during sea urchin development, we examined the expression and function of the Hemicentrotus pulcherrimus homologs of Sumf1 and Sumf2 (HpSumf1 and HpSumf2, respectively). Expression of HpSumf1 but not HpSumf2 mRNA was dynamically changed during early development. Functional analyses of recombinant HpSumf1 and HpSumf2 using HEK293T cells expressing mouse arylsulfatase A (ArsA) indicated that HpSumf1 and HpSumf2 were both able to activate mammalian ArsA. Knockdown of HpSumf1 using morpholino antisense oligonucleotides caused abnormal spicule formation in the sea urchin embryo. Injection of HpSumf2 mRNA had no effect on skeletogenesis, while injection of HpSumf1 mRNA induced severe supernumerary spicule formation. Taken together, these findings suggest that HpSumf1 is involved in the activation of sulfatases required for control of skeletogenesis.
Starting Page	157
Ending Page	166
Page Count	10
File Format	PDF
ISSN	0949944X
Journal	Development Genes and Evolution
Volume Number	221
Issue Number	3
e-ISSN	1432041X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-06-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sulfatase Sulfatase modifying factor Skeletogenesis Sea urchin Biochemistry Cell Biology Animal Genetics and Genomics Developmental Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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