NDLI: Identification and expression analysis of a doublesex1 gene in Daphnia pulex during different reproductive stages

Content Provider	Springer Nature Link
Author	Xu, Shan Liang Zhou, Wei Chen, Ping Zhou, Jian Kai Zou, Xiu Wang, Chun Lin Wang, Dan Li Zhao, Yun Long
Copyright Year	2014
Abstract	The gene doublesex (dsx) has shown deep conservation in the sex determination in many organisms. Environmental stimuli initiate a switch in the reproductive strategy of Daphnia pulex from asexual to sexual reproduction; however, occasionally, changes in environmental conditions will not lead to this transition. So study genetic responses to environmental stimuli and the molecular basis for the switch of reproductive stages are urgently needed. Therefore, we isolated and sequenced a D. pulex doublesex1 gene (Dpdsx1) and analyzed its expression and location by quantitative polymerase chain reaction (qPCR) and whole-mount in situ hybridization in D. pulex during different stages of reproduction. The predicted amino acid sequence has 335 amino acids that contained one DM domain and one dimerization domain, which is characteristic of insect orthologs of Dsx. Real-time PCR showed that Dpdsx1 expression decreased significantly (P < 0.05) in different reproductive stages in the following order: male, parthenogenetic female, ephippial female, resting egg, and juvenile female. Whole-mount in situ hybridization revealed that Dpdsx1 is expressed in the first antennae, first thoracic limb and compound eye in males, whereas expression levels in the corresponding sites of parthenogenetic and ephippial females were relatively weak. Dpdsx1 could not be detected in the gonads of males or ephippial and parthenogenetic females. Taken together, these different reproductive stages’ and sex specific expression patterns are regulated temporally and spatially. We speculate that Dpdsx1 may involve in switching different stages of reproduction and in sexual differentiation in D. pulex.
Starting Page	147
Ending Page	157
Page Count	11
File Format	PDF
ISSN	0949944X
Journal	Development Genes and Evolution
Volume Number	224
Issue Number	3
e-ISSN	1432041X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-05-29
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Daphnia pulex Doublesex1 Gene expression Reproduction switching Whole-mount in situ hybridization Developmental Biology Neurosciences Cell Biology Biochemistry Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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