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| Content Provider | Springer Nature Link |
|---|---|
| Author | Salemme, Marinella Sica, Maria Gaudio, Lucia Aceto, Serena |
| Copyright Year | 2011 |
| Abstract | The class B MADS-box genes belong to two distinct functional groups: the AP3/DEF-like and the PI/GLO-like sub-families. In orchids, AP3/DEF-like genes are present in four copies, each with a different role in floral organ formation, which is described in the “orchid code” model. Interestingly, the orchid PI/GLO-like genes are present in two copies in Orchidinae, whereas they are described as single copy in the other orchid lineages. The two PI/GLO-like paralogs have site-specific different selective constraints; in addition, they show relaxation of purifying selection when compared to the single-copy lineages. In this study, we present a comparative analysis of the expression patterns of the two PI/GLO-like paralogs, OrcPI and OrcPI2, in floral tissues of Orchis italica in different developmental stages using real-time PCR. The two genes show similar expression profiles in the tissue examined, with differences detectable between immature and mature inflorescence. In all cases, OrcPI2 is expressed at a higher level than OrcPI. Real-time PCR results reveal that the co-expression of the two duplicated loci could have a fully or partially redundant function. The possible evolutionary fate of OrcPI and OrcPI2 is discussed as well as their involvement in ovary development. |
| Starting Page | 241 |
| Ending Page | 246 |
| Page Count | 6 |
| File Format | |
| ISSN | 0949944X |
| Journal | Development Genes and Evolution |
| Volume Number | 221 |
| Issue Number | 4 |
| e-ISSN | 1432041X |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-09-04 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Expression pattern Gene duplication MADS-box genes Real-time RT-PCR Animal Genetics and Genomics Biochemistry Developmental Biology Cell Biology Neurosciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Developmental Biology |
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