NDLI: A putative ABC transporter gene, ZRA1, is required for zearalenone production in Gibberella zeae

Content Provider	Springer Nature Link
Author	Lee, Seunghoon Son, Hokyoung Lee, Jungkwan Lee, Ye Ryun Lee, Yin Won
Copyright Year	2011
Abstract	Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the cluster. In this study, we performed microarray analyses from the wild-type strain with and without ZEA supplementation and ZEA-nonproducing strain zeb2 to discover other genes responsible for ZEA biosynthesis. Three putative ABC transporters were significantly down-regulated in the zeb2 and were under positive regulation of the ZEB2 gene, which functions as a transcriptional activator for ZEA production in this fungus. However, only one gene (ZRA1) was found to be up-regulated by 20-fold in the wild-type strain supplemented with ZEA, and deletion of ZRA1 resulted in reduced ZEA production. Deletions of the other two genes showed similar ZEA productions as the wild-type strain. ZRA1 localized to the plasma membrane and vacuoles indicating possible roles of ZRA1 as a transporter. This study indicated that ZRA1 is involved in ZEA production and shares a common regulatory mode with ZEA cluster genes by ZEB2.
Starting Page	343
Ending Page	351
Page Count	9
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	57
Issue Number	5
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-08-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ABC transporters Fusarium graminearum Gibberella zeae Zearalenone Plant Sciences Microbiology Proteomics Biochemistry Microbial Genetics and Genomics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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