NDLI: Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases

Content Provider	Springer Nature Link
Author	Ruprich Robert, Gwenaël Wery, Maxime Després, Daphné Boulard, Yves Thuriaux, Pierre
Copyright Year	2011
Abstract	The catalytic center of yeast RNA polymerase II and III contains an acidic loop borne by their second largest subunit (Rpb2-832GYNQED837, Rpc128-764GYDIED769) and highly conserved in all cellular and viral DNA-dependent RNA polymerases. A site-directed mutagenesis of this dicarboxylic motif reveals its strictly essential character in RNA polymerase III, with a slightly less stringent pattern in RNA polymerase II, where rpb2-E836Q and other substitutions completely prevent growth, whereas rpb2-E836A combines a dominant growth defect with severe lethal sectoring. A mild but systematic increase in RNA polymerase occupancy and a strict dependency on the transcript cleavage factor TFIIS (Dst1) also suggest a slower rate of translocation or higher probability of transcriptional stalling in this mutation. A conserved nucleotide triphosphate funnel domain binds the Rpb2-832GYNQED837 loop by an Rpb2-R1020/Rpb2-D837 salt-bridge. Molecular dynamic simulations reveal a second bridge (Rpb1-K752/Rpb2-E836), which may account for the critical role of the invariant Rpb2-E836. Rpb2-E836 and the funnel domain are not found among the RNA-dependent eukaryotic RNA polymerases and may thus represent a specific adaptation to double-stranded DNA templates.
Starting Page	327
Ending Page	334
Page Count	8
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	57
Issue Number	5
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Saccharomyces cerevisiae RNA polymerase Baculovirus TFIIS DST1 Plant Sciences Microbiology Proteomics Biochemistry Microbial Genetics and Genomics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Polyphosphate: popping up from oblivion

Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae

Carnitine and carnitine acetyltransferases in the yeast Saccharomyces cerevisiae: a role for carnitine in stress protection

A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition

Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae

Prion variant maintained only at high levels of the Hsp104 disaggregase

The distribution of inverted repeat sequences in the Saccharomyces cerevisiae genome

Reprogramming of nonfermentative metabolism by stress-responsive transcription factors in the yeast Saccharomyces cerevisiae

Negative epistasis: a route to intraspecific reproductive isolation in yeast?

Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases

Similar Documents

Polyphosphate: popping up from oblivion

Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae

Carnitine and carnitine acetyltransferases in the yeast Saccharomyces cerevisiae: a role for carnitine in stress protection

A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition

Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae

Prion variant maintained only at high levels of the Hsp104 disaggregase

The distribution of inverted repeat sequences in the Saccharomyces cerevisiae genome

Reprogramming of nonfermentative metabolism by stress-responsive transcription factors in the yeast Saccharomyces cerevisiae

Negative epistasis: a route to intraspecific reproductive isolation in yeast?

Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases