NDLI: Identification and analysis of Phytophthora cactorum genes up-regulated during cyst germination and strawberry infection

Content Provider	Springer Nature Link
Author	Chen, Xiaoren Klemsdal, Sonja Sletner Brurberg, May Bente
Copyright Year	2011
Abstract	The oomycete Phytophthora cactorum can cause economically important diseases on numerous host plants worldwide, such as crown rot on strawberry. To explore the molecular mechanisms underlying the pathogenicity of P. cactorum on strawberry, transcriptional analysis of P. cactorum during strawberry infection and cyst germination was performed by applying suppression subtractive hybridization (SSH) and effector-specific differential display (ESDD) techniques. Two SSH cDNA libraries were generated, enriched for P. cactorum genes expressed during infection or during cyst germination, respectively, and 137 unique differentially expressed genes were identified. To specifically select RxLR effector genes from P. cactorum, ESDD was performed using RxLR and EER motif-based degenerate primers. Eight RxLR effector candidate genes as well as 67 other genes were identified out of 124 selected fragments. The expression levels of 20 putatively up-regulated genes were further analyzed using real-time RT-PCR, showing that, indeed 19 of these 20 genes were up-regulated during at least one of the studied developmental stages or during strawberry crown invasion, relative to the mycelium. This study provides a first overview of P. cactorum genes that are up-regulated immediately prior to or during strawberry infection and also provides a novel method for selecting RxLR effector genes from the unsequenced genome of P. cactorum.
Starting Page	297
Ending Page	315
Page Count	19
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	57
Issue Number	5
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-06-23
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phytophthora cactorum Strawberry RxLR effectors SSH Differential display Real-time PCR Plant Sciences Microbiology Proteomics Biochemistry Microbial Genetics and Genomics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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