NDLI: Enhancement of volatile thiol release of Saccharomyces cerevisiae strains using molecular breeding

Content Provider	Springer Nature Link
Author	Dufour, Matthieu Zimmer, Adrien Thibon, Cécile Marullo, Philippe
Copyright Year	2013
Abstract	Cysteine-conjugated volatile thiols are powerful aromatic compounds that contribute to the fruity notes of many white wines and especially Sauvignon Blanc. Genetic selection programs of wine yeast starters able to produce more volatile thiols constitute, therefore, an important goal for the wine industry. Recent investigations on yeast metabolism suggested that the ß-lyase Irc7p and the control of its gene expression by nitrogen catabolite repression constitute a rational way for yeast genetic improvement. This work demonstrates that the use of a natural ure2 mutation can be used to design wine starters with an enhanced capacity of volatile thiols production. By applying backcrosses driven by molecular markers, this allelic form was introduced in different starter backgrounds. Our investigations demonstrate that the ure2 inheritance is able to enhance the production of 4MMP (recently renamed 4MSP) and 3MH (recently renamed 3SH). For 4MMP, this effect depends of the presence of the allele IRC7 LT encoding a long form of the Irc7 protein. Moreover, a correlation in between the expression level of this allelic form and 4MMP production was found within industrial starters. All together, these results emphasised the use of molecular breeding for improving quantitative traits of industrial strains without the use of genetically modifying strategies.
Starting Page	5893
Ending Page	5905
Page Count	13
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	13
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-02-20
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	S. cerevisiae IRC7 URE2 Volatile thiols Molecular breeding Wine yeast Biotechnology Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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