NDLI: Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source

Content Provider	Springer Nature Link
Author	da, Shuhei Kawai, Yoshifumi Miyazaki, Takaya Tanaka, Tsutomu Kondo, Akihiko
Copyright Year	2013
Abstract	We screened for high-activity endoglucanase (EG) as a first step toward the creation of cellulose-assimilating Streptomyces lividans transformants. EGs derived from Thermobifida fusca YX, Tfu0901, and S. lividans, cellulase B (CelB), were successfully expressed. Genes encoding Tfu0901 or CelB were introduced into S. lividans using the integrative vector pTYM18 and the high-copy-number vector pUC702, and EG activity was detected in the supernatant of each transformant. To achieve coexpression of EG and transglutaminase, the transglutaminase gene was introduced into EG-secreting S. lividans using pUC702. S. lividans coexpressing EG and transglutaminase effectively assimilated phosphoric acid swollen cellulose. The yield of Streptomyces cinnamoneus transglutaminase in the culture supernatant was 7.2 mg/L, which was 18 times higher than that of the control strain. To demonstrate the versatility of our system, we also created an EG-producing S. lividans transformant capable of coexpressing endoxylanase. The EG-secreting S. lividans transformants constructed here can be used to produce other useful compounds through cellulose fermentation.
Starting Page	5711
Ending Page	5720
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	13
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-04-21
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Endoglucanase Streptomyces Cellulose Transglutaminase Endoxylanase Biotechnology Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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