NDLI: Changes in biofilm structure during the colonization of chalcopyrite by Acidithiobacillus thiooxidans

Content Provider	Springer Nature Link
Author	García Meza, J. V. Fernández, J. J. Lara, R. H. González, I.
Copyright Year	2012
Abstract	Biofilms of Acidithiobacillus thiooxidans were grown on the surface of massive chalcopyrite electrodes (MCE) where different secondary sulfur phases were previously formed by potentiostatic oxidation of MCE at 0.780 ≤ E an ≤ 0.965 V (electrooxidized MCE, eMCE). The formation of mainly S0 and minor amounts of CuS and S n 2− were detected on eMCEs. The eMCEs were incubated with A. thiooxidans cells for 1, 12, 24, 48, and 120 h in order to temporally monitor changes in eMCE's secondary phases, biofilm structure, and extracellular polymeric substance (EPS) composition (lipids, proteins, and polysaccharides) using microscopic, spectroscopic, electrochemical, and biochemical techniques. The results show significant cell attachments with stratified biofilm structure since the first hour of incubation and EPS composition changes, the most important being production after 48–120 h when the highest amount of lipids and proteins were registered. During 120 h, periodic oxidation/formation of S0/S n 2− was recorded on biooxidized eMCEs, until a stable CuS composition was formed. In contrast, no evidence of CuS formation was observed on the eMCEs of the abiotic control, confirming that CuS formation results from microbial activity. The surface transformation of eMCE induces a structural transformation of the biofilm, evolving directly to a multilayered biofilm with more hydrophobic EPS and proteins after 120 h. Our results suggest that A. thiooxidans responded to the spatial and temporal distribution and chemical reactivity of the S n 2−/S0/CuS phases throughout 120 h. These results suggested a strong correlation between surface speciation, hydrophobic domains in EPS, and biofilm organization during chalcopyrite biooxidation by A. thiooxidans.
Starting Page	6065
Ending Page	6075
Page Count	11
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	13
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-09-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Acidithiobacillus thiooxidans Chalcopyrite Biofilms Electrooxidation Interfacial analysis Biotechnology Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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