NDLI: Molecular genotype identification of the Gallus gallus major histocompatibility complex

Content Provider	Springer Nature Link
Author	Fulton, Janet E. Juul Madsen, Helle R. Ashwell, Christopher M. McCarron, Amy M. Arthur, James A. O’Sullivan, Neil P. Taylor, Robert L.
Copyright Year	2006
Abstract	The chicken major histocompatibility complex (MHC) is commonly defined by serologic reactions of erythrocytes with antibodies specific to the highly polymorphic MHC class I (BF) and MHC class IV (BG) antigens. The microsatellite marker LEI0258 is known to be physically located within the MHC, between the BG and BF regions. DNA from various serologically defined MHC haplotypes was amplified by polymerase chain reaction with primers surrounding this marker. Twenty-six distinctive allele sizes were identified. Some serologically well-defined MHC haplotypes shared a common LEI0258 allele size but could be distinguished either by the addition of information from another nearby marker (MCW0371) or by small indels or single nucleotide polymorphism (SNP) differences between the alleles. The association between LEI0258 allele and serologically defined MHC haplotype was very consistent for the same haplotype from multiple sources. Sequence information for the region defined by LEI0258 was obtained for 51 different haplotypes. Two internal repeats whose lengths were 13 and 12 bp, respectively, are the primary basis for allelic variability. Allele size variation ranges from 182 to 552 bp. Four indels and five SNPs in the surrounding sequence provide additional means for distinguishing alleles. Typing with LEI0258 and MCW0371 will be useful in identifying MHC haplotypes in outbred populations of chickens particularly for the initial development of serological reagents.
Starting Page	407
Ending Page	421
Page Count	15
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	58
Issue Number	5-6
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-05-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chicken MHC Molecular identification MHC haplotype Microsatellite Genotype sequence Allergology Cell Biology Gene Function Human Genetics Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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