NDLI: Survey of major histocompatibility complex class II diversity in pig-tailed macaques

Content Provider	Springer Nature Link
Author	Karl, Julie A. Heimbruch, Katelyn E. Vriezen, Claire E. Mironczuk, Cassandra J. Dudley, Dawn M. Wiseman, Roger W. O’Conr, David H.
Copyright Year	2014
Abstract	Pig-tailed macaques (Macaca nemestrina) serve as important models for human infectious disease research. Major histocompatibility complex (MHC) class II molecules are important to this research since they present peptides to CD4+ T cells. Despite the importance of characterizing the MHC-II alleles expressed in model species like pig-tailed macaques, to date, less than 150 MHC-II alleles have been named for the six most common classical class II loci (DRA, DRB, DQA, DQB, DPA, and DPB) in this population. Additionally, only a small percentage of these alleles are full-length, making it impossible to use the known sequence for reagent development. To address this, we developed a fast, high-throughput method to discover full-length MHC-II alleles and used it to characterize alleles in 32 pig-tailed macaques. By this method, we identified 128 total alleles across all six loci. We also performed an exon 2-based genotyping assay to validate the full-length sequencing results; this genotyping assay could be optimized for use in determining MHC-II allele frequencies in large cohorts of pig-tailed macaques.
Starting Page	613
Ending Page	623
Page Count	11
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	66
Issue Number	11
e-ISSN	14321211
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-08-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Macaca nemestrina Major histocompatibility complex MHC class II alleles Next-generation sequencing Immunology Human Genetics Gene Function Cell Biology Allergology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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