NDLI: Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing : Mafa-class I polymorphism

Content Provider	Springer Nature Link
Author	Shiina, Takashi Yamada, Yukiho Aarnink, Alice Suzuki, Shingo Masuya, Anri Ito, Sayaka Ido, Daisuke Yamanaka, Hisashi Iwatani, Chizuru Tsuchiya, Hideaki Ishigaki, Hirohito Itoh, Yasushi Ogasawara, Kazumasa Kulski, Jerzy K. Blancher, Antoine
Copyright Year	2015
Abstract	Although the low polymorphism of the major histocompatibility complex (MHC) transplantation genes in the Filipino cynomolgus macaque (Macaca fascicularis) is expected to have important implications in the selection and breeding of animals for medical research, detailed polymorphism information is still lacking for many of the duplicated class I genes. To better elucidate the degree and types of MHC polymorphisms and haplotypes in the Filipino macaque population, we genotyped 127 unrelated animals by the Sanger sequencing method and high-resolution pyrosequencing and identified 112 different alleles, 28 at cynomolgus macaque MHC (Mafa)-A, 54 at Mafa-B, 12 at Mafa-I, 11 at Mafa-E, and seven at Mafa-F alleles, of which 56 were newly described. Of them, the newly discovered Mafa-A8*01:01 lineage allele had low nucleotide similarities (<86 %) with primate MHC class I genes, and it was also conserved in the Vietnamese and Indonesian populations. In addition, haplotype estimations revealed 17 Mafa-A, 23 Mafa-B, and 12 Mafa-E haplotypes integrated with 84 Mafa-class I haplotypes and Mafa-F alleles. Of these, the two Mafa-class I haplotypes, F/A/E/B-Hp1 and F/A/E/B-Hp2, had the highest haplotype frequencies at 10.6 and 10.2 %, respectively. This suggests that large scale genetic screening of the Filipino macaque population would identify these and other high-frequency Mafa-class I haplotypes that could be used as MHC control animals for the benefit of biomedical research.
Starting Page	563
Ending Page	578
Page Count	16
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	67
Issue Number	10
e-ISSN	14321211
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-09-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cynomolgus macaque Major histocompatibility complex MHC Polymorphism Haplotype Next generation sequencing NGS Sanger sequencing Immunology Human Genetics Gene Function Cell Biology Allergology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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Diversity of MHC class I haplotypes in cynomolgus macaques

Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing

Study of MHC class II region polymorphism in the Filipino cynomolgus macaque population

Major histocompatibility complex haplotyping and long-amplicon allele discovery in cynomolgus macaques from Chinese breeding facilities

Characterization of 47 MHC class I sequences in Filipino cynomolgus macaques

Comprehensive identification of high-frequency and co-occurring Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA alleles in Vietnamese cynomolgus macaques

Definition of Mafa-A and -B haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis)

Definition of Mafa-A and -B haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis)

Multilocus definition of MHC haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis)

Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing : Mafa-class I polymorphism

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Diversity of MHC class I haplotypes in cynomolgus macaques

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Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing : Mafa-class I polymorphism