NDLI: Identification of novel MHC class I sequences in pig-tailed macaques by amplicon pyrosequencing and full-length cDNA cloning and sequencing

Content Provider	Springer Nature Link
Author	O’Leary, Claire E. Wiseman, Roger W. Karl, Julie A. Bimber, Benjamin N. Lank, Simon M. Tuscher, Jennifer J. O’Conr, David H.
Copyright Year	2009
Abstract	Pig-tailed macaques (Macaca nemestrina) provide important animal models in biomedical research, but utility of this species for HIV and other disease pathogenesis research is limited by incomplete knowledge of major histocompatibility complex (MHC) class I genetics. Here, we describe comprehensive MHC class I genotyping of 24 pig-tailed macaques, using pyrosequencing to evaluate a 367- bp complementary DNA (cDNA)-PCR amplicon spanning the highly polymorphic peptide-binding region of MHC class I transcripts. We detected 29 previously described Mane transcripts, 90 novel class I sequences, and eight shared MHC class IB haplotypes. We used this genotyping data to inform full-length MHC class I cDNA allele discovery, characterizing 66 novel full-length transcripts. These new full-length sequences nearly triple the number of Mane-B cDNA sequences previously characterized. The comprehensive genotypes and full-length Mane transcripts described herein add value to pig-tailed macaques as model organisms in biomedical research; furthermore, the coordinated method for MHC genotyping and allele discovery is extensible to other less well-characterized nonhuman primate species.
Starting Page	689
Ending Page	701
Page Count	13
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	61
Issue Number	10
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-09-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Macaca nemestrina Pig-tailed macaques MHC Pyrosequencing Allergology Gene Function Cell Biology Human Genetics Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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