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| Content Provider | Springer Nature Link |
|---|---|
| Author | Williams, P. Stephen |
| Copyright Year | 2016 |
| Abstract | Asymmetrical flow field-flow fractionation (As-FlFFF) is a widely used technique for analyzing polydisperse nanoparticle and macromolecular samples. The programmed decay of cross flow rate is often employed. The interdependence of the cross flow rate through the membrane and the fluid flow along the channel length complicates the prediction of elution time and fractionating power. The theory for their calculation is presented. It is also confirmed for examples of exponential decay of cross flow rate with constant channel outlet flow rate that the residual sample polydispersity at the channel outlet is quite well approximated by the reciprocal of four times the fractionating power. Residual polydispersity is of importance when online MALS or DLS detection are used to extract quantitative information on particle size or molecular weight. The theory presented here provides a firm basis for the optimization of programmed flow conditions in As-FlFFF. Graphical abstract Channel outlet polydispersity remains significant following fractionation by As-FlFFF under conditions of programmed decay of cross flow rate |
| Starting Page | 317 |
| Ending Page | 334 |
| Page Count | 18 |
| File Format | |
| ISSN | 16182642 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume Number | 409 |
| Issue Number | 1 |
| e-ISSN | 16182650 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-11-12 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Asymmetrical flow field-flow fractionation Programmed operation Fractionating power Outlet stream polydispersity Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Biochemistry |
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