NDLI: Aptamer-based surface-enhanced Raman scattering (SERS) sensor for thrombin based on supramolecular recognition, oriented assembly, and local field coupling

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Aptamer-based surface-enhanced Raman scattering (SERS) sensor for thrombin based on supramolecular recognition, oriented assembly, and local field coupling

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Aptamer-based surface-enhanced Raman scattering (SERS) sensor for thrombin based on supramolecular recognition, oriented assembly, and local field coupling

Content Provider	Springer Nature Link
Author	Yang, Liyuan Fu, Cuicui Wang, Hailong Xu, Shuping Xu, Weiqing
Copyright Year	2016
Abstract	A supramolecular recognition and oriented assembly system was developed on chip for the highly selective surface-enhanced Raman scattering (SERS) detection of thrombin by means of the aptamer-based SERS tag method. A 15-base thrombin-binding aptamer (TBA15) with a thiol end was first immobilized on an Ag nanoprism array by the S–Ag bond. This aptamer has high binding affinity with thrombin when it folds into a G-quadruplex structure. After the recognition between the aptamer and thrombin, a bridge is built between the SERS tag (4-mercaptobenzoic acid marked Ag nanoparticle) and the fixed thrombin based on the activation of the carboxylic group of 4-mercaptobenzoic acid. Thus, the quantitative detection of thrombin can be achieved based on the SERS intensity of the immobilized SERS tags. The obvious advantages of this sensing method are as follows: (1) remarkable SERS enhancement due to the high electric field coupling effect via the gap structure formation, which improves the sensitivity of the SERS detection and the limit of detection of this method arrives in 1.6 × 10–11 M, (2) high selectivity based on the specific aptamer recognition toward thrombin, which can be extended to other enzymes easily by changing a proper sequence, (3) high repeatability of SERS signals according to a highly ordered structure, and (4) highly efficient oriented assembly of a sandwich structure over an Ag nanoprism array. The proposed method is expected to be a practical implement in medical diagnosis. Graphical Abstract Illustration of the aptamer-based SERS sensor for thrombin detection
Starting Page	235
Ending Page	242
Page Count	8
File Format	PDF
ISSN	16182642
Journal	Analytical and Bioanalytical Chemistry
Volume Number	409
Issue Number	1
e-ISSN	16182650
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-10-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thrombin SERS Local field coupling Oriented assembly Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Biochemistry

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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