NDLI: A new analytical platform based on field-flow fractionation and olfactory sensor to improve the detection of viable and non-viable bacteria in food

Content Provider	Springer Nature Link
Author	Roda, Barbara Mirasoli, Mara Zattoni, Andrea Casale, Monica Oliveri, Paolo Bigi, Alessandro Reschiglian, Pierluigi Simoni, Patrizia Roda, Aldo
Copyright Year	2016
Abstract	An integrated sensing system is presented for the first time, where a metal oxide semiconductor sensor-based electronic olfactory system (MOS array), employed for pathogen bacteria identification based on their volatile organic compound (VOC) characterisation, is assisted by a preliminary separative technique based on gravitational field-flow fractionation (GrFFF). In the integrated system, a preliminary step using GrFFF fractionation of a complex sample provided bacteria-enriched fractions readily available for subsequent MOS array analysis. The MOS array signals were then analysed employing a chemometric approach using principal components analysis (PCA) for a first-data exploration, followed by linear discriminant analysis (LDA) as a classification tool, using the PCA scores as input variables. The ability of the GrFFF-MOS system to distinguish between viable and non-viable cells of the same strain was demonstrated for the first time, yielding 100 % ability of correct prediction. The integrated system was also applied as a proof of concept for multianalyte purposes, for the detection of two bacterial strains (Escherichia coli O157:H7 and Yersinia enterocolitica) simultaneously present in artificially contaminated milk samples, obtaining a 100 % ability of correct prediction. Acquired results show that GrFFF band slicing before MOS array analysis can significantly increase reliability and reproducibility of pathogen bacteria identification based on their VOC production, simplifying the analytical procedure and largely eliminating sample matrix effects. The developed GrFFF-MOS integrated system can be considered a simple straightforward approach for pathogen bacteria identification directly from their food matrix. Graphical abstract An integrated sensing system is presented for pathogen bacteria identification in food, in which field-flow fractionation is exploited to prepare enriched cell fractions prior to their analysis by electronic olfactory system analysis
Starting Page	7367
Ending Page	7377
Page Count	11
File Format	PDF
ISSN	16182642
Journal	Analytical and Bioanalytical Chemistry
Volume Number	408
Issue Number	26
e-ISSN	16182650
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-08-13
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electronic olfactory system Field-flow fractionation Metal oxide semiconductor (MOS) sensor array Pathogen bacteria Viable and non-viable bacteria Chemometrics Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter

Field flow fractionation techniques to explore the “nano-world”

Fractionating power and outlet stream polydispersity in asymmetrical flow field-flow fractionation. Part II: programmed operation

Video approach to chemiluminescence detection using a low-cost complementary metal oxide semiconductor (CMOS)-based camera: determination of paracetamol in pharmaceutical formulations

Metal Oxide Nanorods-Based Sensor Array for Selective Detection of Biomarker Gases

Can slip walls improve field-flow fractionation or hydrodynamic chromatography?

Study on graphene metal oxide semiconductor nanocomposite based sensors for detection of volatile organic compounds at low temperature

Use of chemometrics to optimize a glucose assay on a paper microfluidic platform

Role of the shape of various bacteria in their separation by Microthermal Field-Flow Fractionation.

A new analytical platform based on field-flow fractionation and olfactory sensor to improve the detection of viable and non-viable bacteria in food

Similar Documents

Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter

Field flow fractionation techniques to explore the “nano-world”

Fractionating power and outlet stream polydispersity in asymmetrical flow field-flow fractionation. Part II: programmed operation

Video approach to chemiluminescence detection using a low-cost complementary metal oxide semiconductor (CMOS)-based camera: determination of paracetamol in pharmaceutical formulations

Metal Oxide Nanorods-Based Sensor Array for Selective Detection of Biomarker Gases

Can slip walls improve field-flow fractionation or hydrodynamic chromatography?

Study on graphene metal oxide semiconductor nanocomposite based sensors for detection of volatile organic compounds at low temperature

Use of chemometrics to optimize a glucose assay on a paper microfluidic platform

Role of the shape of various bacteria in their separation by Microthermal Field-Flow Fractionation.

A new analytical platform based on field-flow fractionation and olfactory sensor to improve the detection of viable and non-viable bacteria in food