NDLI: Label-free electrochemical genosensor based on mesoporous silica thin film

Content Provider	Springer Nature Link
Author	Saadaoui, Maroua Fernández, Iñigo Luna, Gema Díez, Paula Campuza, Susana Raouafi, ureddine Sánchez, Alfredo Pingarrón, José M. Villalonga, Reynaldo
Copyright Year	2016
Abstract	A novel label-free electrochemical strategy for nucleic acid detection was developed by using gold electrodes coated with mesoporous silica thin films as sensing interface. The biosensing approach relies on the covalent attachment of a capture DNA probe on the surface of the silica nanopores and further hybridization with its complementary target oligonucleotide sequence, causing a diffusion hindering of an Fe(CN)6 3−/4− electrochemical probe through the nanochannels of the mesoporous film. This DNA-mesoporous silica thin film-modified electrodes allowed sensitive (91.7 A/M) and rapid (45 min) detection of low nanomolar levels of synthetic target DNA (25 fmol) and were successfully employed to quantify the endogenous content of Escherichia coli 16S ribosomal RNA (rRNA) directly in raw bacterial lysate samples without isolation or purification steps. Moreover, the 1-month stability demonstrated by these biosensing devices enables their advanced preparation and storage, as desired for practical real-life applications. Graphical abstract Mesoporous silica thin films as scaffolds for the development of novel label-free electrochemical genosensors to perform selective, sensitive and rapid detection of target oligonucleotide sequences. Application towards E. coli determination
Starting Page	7321
Ending Page	7327
Page Count	7
File Format	PDF
ISSN	16182642
Journal	Analytical and Bioanalytical Chemistry
Volume Number	408
Issue Number	26
e-ISSN	16182650
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-05-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genosensor Mesoporous silica film Cyclic voltammetry E. coli 16S rRNA DNA Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples using solid-phase recombinase polymerase amplification

Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products

Extraction of cell-free DNA from urine, using polylysine-coated silica particles

Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

E-DNA sensors for convenient, label-free electrochemical detection of hybridization

Self-assembled monolayer-assisted label-free electrochemical genosensor for specific point-of-care determination of Haemophilus influenzae.

Evaluation of the antibacterial activity of a cationic polymer in aqueous solution with a convenient electrochemical method

A novel toolbox for E. coli lysis monitoring

A sensitive aptasensor based on molybdenum carbide nanotubes and label-free aptamer for detection of bisphenol A

Label-free electrochemical genosensor based on mesoporous silica thin film

Similar Documents

Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples using solid-phase recombinase polymerase amplification

Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products

Extraction of cell-free DNA from urine, using polylysine-coated silica particles

Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

E-DNA sensors for convenient, label-free electrochemical detection of hybridization

Self-assembled monolayer-assisted label-free electrochemical genosensor for specific point-of-care determination of Haemophilus influenzae.

Evaluation of the antibacterial activity of a cationic polymer in aqueous solution with a convenient electrochemical method

A novel toolbox for E. coli lysis monitoring

A sensitive aptasensor based on molybdenum carbide nanotubes and label-free aptamer for detection of bisphenol A

Label-free electrochemical genosensor based on mesoporous silica thin film