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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhang, Tianjiao Zhang, Chuanbao Zhao, Haijian Zeng, Jie Zhang, Jiangtao Zhou, Weiyan Yan, Ying Wang, Yufei Wang, Mo Chen, Wenxiang |
| Copyright Year | 2016 |
| Abstract | Accurate and precise glucose measurements are requisite for ensuring appropriate diagnosis and management of diseases related to hyperglycemia or hypoglycemia. It is necessary to have a higher order method to provide an accuracy base to which routine methods can be compared. We developed and evaluated a highly reliable measurement procedure based on isotope dilution liquid chromatography–tandem mass spectrometry (ID LC-MS/MS) with a simple one-step derivatization. An appropriate amount of serum was accurately weighed and spiked with an isotope-labeled internal standard. After protein precipitation, the supernatant was reacted with 1-phenyl-3-methyl-5-pyrazolone for chemical structural transformation. The glucose derivatives were analyzed with LC-MS/MS in positive electrospray ionization mode. The within-run and total CVs ranged from 0.28 to 0.42 % and from 0.42 to 0.76 %, respectively, for a concentration range of 1.691 to 15.676 mmol/L. A regression comparison of the presented method to an existing RMP based on ID GC-MS showed agreement with no statistical difference (Y = 0.9985X-0.008; 95 % CI for the slope, 0.9966 to 1.001; 95 % CI for the intercept, −0.012 to 0.019). The structural analogs of glucose with a molecular mass of 180 were tested, and no significant interference effect was found. The limit of quantification was estimated to 0.8 ng glucose in absolute amount. This method is accurate, simple, and can serve as a candidate reference measurement procedure (RMP) in the establishment of a serum glucose reference system. |
| Starting Page | 7403 |
| Ending Page | 7411 |
| Page Count | 9 |
| File Format | |
| ISSN | 16182642 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume Number | 408 |
| Issue Number | 26 |
| e-ISSN | 16182650 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-08-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | 1-Phenyl-3-methyl-5-pyrazolone Liquid chromatography-tandem mass spectrometry Serum glucose Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Biochemistry |
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