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Tip-enhanced fluorescence microscopy of high-density samples
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xie, Changan Mu, Chun Mei Cox, Jonathan R. Gerton, Jordan Mitchell |
| Copyright Year | 2006 |
| Abstract | High-density samples of fluorescent quantum dots (QDs) were imaged using an apertureless near-field optical microscopy technique. QD fluorescence was modulated by oscillating a silicon atomic force microscope tip above an illuminated sample and a lock-in amplifier was used to suppress background from the excitation laser. Spatial resolution near 10nm and a peak signal-to-noise ratio (SNR) of ∼60 were achieved. Individual QDs within high-density ensembles were still easily resolved (SNR>5) at a density of 14QDs∕μm2. These results have favorable implications for the eventual nanoscale imaging of viable biological systems, such as cellular membranes. |
| Starting Page | 143117 |
| Ending Page | 143117 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1063/1.2358122 |
| Alternate Webpage(s) | http://www.physics.utah.edu/people/faculty/gerton/Xie_APL_2006.pdf |
| Alternate Webpage(s) | http://www.physics.utah.edu/~gertonlab/pdfs/xieAppPhysRev2006.pdf |
| Alternate Webpage(s) | http://biophys.w3.kanazawa-u.ac.jp/References/Tip-enhanced-fluorescence/APL-Xie-2006.pdf |
| Alternate Webpage(s) | http://www.s.kanazawa-u.ac.jp/phys/biophys/References/Tip-enhanced-fluorescence/APL-Xie-2006.pdf |
| Alternate Webpage(s) | https://doi.org/10.1063/1.2358122 |
| Volume Number | 89 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
