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Fluorescence quenching in tip-enhanced nonlinear optical microscopy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Krug, John T. Sanchez, Erik De Jesus Xie, X. Sunney |
| Copyright Year | 2005 |
| Abstract | We describe the theoretical treatment of fluorescence quenching in tip-enhanced nonlinear optical microscopy (TENOM). Finite difference time domain simulations demonstrate that while sharp pyramidal probes yield fluorescence signal enhancement that decays monotonically as a function of probe-fluorophore distance, more commonly used conical probes cause more complex image contrast. Fluorescence quenching can thus explain the halo-type images that are sometimes observed in TENOM. Formation of a dielectric spacer layer on the TENOM probe should alleviate the complications associated with quenching. |
| Starting Page | 233102 |
| Ending Page | 233102 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1063/1.1935769 |
| Volume Number | 86 |
| Alternate Webpage(s) | https://bernstein.harvard.edu/papers/krug_apl_2005.pdf |
| Alternate Webpage(s) | http://bernstein.harvard.edu/papers/krug_apl_2005.pdf |
| Alternate Webpage(s) | https://doi.org/10.1063/1.1935769 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
