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Resolving single fluorophores within dense ensembles: contrast limits of tip-enhanced fluorescence microscopy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mangum, Benjamin D. Mu, Chun Mei Gerton, Jordan Mitchell |
| Copyright Year | 2008 |
| Abstract | We investigate the limits of one-photon fluorescence as a contrast mechanism in nanoscale-resolution tip-enhanced optical microscopy. Specifically, we examine the magnitude of tip-induced signal enhancement needed to resolve individual fluorophores within densely-packed ensembles. Modulation of fluorescence signals induced by an oscillating tip followed by demodulation with a lock-in amplifier increases image contrast by nearly two orders of magnitude. A theoretical model of this simple modulation/ demodulation scheme predicts an optimal value for the tip-oscillation amplitude that agrees with experimental measurements. Further, as an important step toward the eventual application of tip-enhanced fluorescence microscopy to the nanoscale structural analysis of biomolecular systems, we show that requisite signal enhancement factors are within the capabilities of commercially available silicon tips. |
| File Format | PDF HTM / HTML |
| DOI | 10.1364/OE.16.006183 |
| PubMed reference number | 18545320 |
| Journal | Medline |
| Volume Number | 16 |
| Issue Number | 9 |
| Alternate Webpage(s) | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/1842EBFCF7B73124ED97128EC4B75B0C/S1431927611001012a.pdf/resolving_single_fluorophores_within_dense_ensembles_contrast_limits_of_tipenhanced_fluorescence_microscopy.pdf |
| Alternate Webpage(s) | http://www.physics.utah.edu/~gertonlab/pdfs/mangumOptics2008.pdf |
| Alternate Webpage(s) | http://biophys.w3.kanazawa-u.ac.jp/References/Tip-enhanced-fluorescence/Microsc-2011.pdf |
| Alternate Webpage(s) | http://www.physics.utah.edu/people/faculty/gerton/Mangum_Opt_Expr_2008.pdf |
| Alternate Webpage(s) | https://doi.org/10.1364/OE.16.006183 |
| Journal | Optics express |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
