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Diagnostic features of transfusion associated graft versus host disease.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Appleton, Anne L. Sviland, Lisbet Pearson, Andrew D. J. Wilkes, Jason Green, Margaret A. Malcolm, Andrew J. |
| Copyright Year | 1994 |
| Abstract | AIMS To define the immunopathological profile of transfusion associated graft versus host disease (TA-GvHD) to elucidate its pathophysiology and to determine if any features are of diagnostic value. METHODS Nine patients (age range 14-61 years) who developed histologically confirmed TA-GvHD between 1989 and 1992 were studied. Immunohistochemical analysis of frozen and formalin fixed skin biopsy tissue was performed. Sections were stained with antibodies to CD3, CD8, CD4 and HLA-DR, using a routine streptavidin-biotin technique with standard diaminobenzidine development. RESULTS All biopsy specimens showed aberrant positive expression of HLA-DR by epidermal keratinocytes. In four patients, all of whom died, HLA-DR was diffusely expressed throughout the epidermis; in the other five cases keratinocyte expression of HLA-DR was more focal. In all biopsy specimens T cells had infiltrated the dermis and epidermis. In all nine cases CD4+ T helper/inducer cells were the predominant T cells. DISCUSSION Immunohistochemical studies are of value in the diagnosis of TA-GvHD. Aberrant keratinocyte expression of HLA-DR and dermal and epidermal infiltration of CD4+ T cells are immunopathological features of TA-GvHD. Immunohistochemical analysis of skin biopsy tissue using antibodies to these markers is thus a useful investigation in pancytopenic patients presenting with unexplained rashes. |
| Starting Page | 650 |
| Ending Page | 656 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcp.bmj.com/content/jclinpath/47/6/541.full.pdf |
| PubMed reference number | 8063938v1 |
| Volume Number | 47 |
| Issue Number | 6 |
| Journal | Journal of clinical pathology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biopsy of skin procedure Cessation of life Dermis Epidermis Exanthema Formalin Graft versus host disease prophylaxis/therapy Graft-vs-Host Disease HLA-DR Antigens Histocompatibility Antigens Class II Infiltration KRAS NM_004985.3:c.34_35delGGinsTA Patients Somatoform Disorder Specimen Streptavidin human papillomavirus vaccine, TA keratinocyte |
| Content Type | Text |
| Resource Type | Article |
