NDLI: Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection† †Electronic supplementary information (ESI) available: Materials and methods, compound characterisation, data for ligation reactions and miRNA detection. See DOI: 10.1039/c7sc02736b

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Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection† †Electronic supplementary information (ESI) available: Materials and methods, compound characterisation, data for ligation reactions and miRNA detection. See DOI: 10.1039/c7sc02736b

Content Provider	Semantic Scholar
Author	Sayers, Jessica Payne, Richard J. Winssinger, Nicolas
Copyright Year	2018
Abstract	The development of a rapid and chemoselective selenocystine-selenoester peptide ligation that operates at nanomolar reactant concentrations has been developed by utilising PNA templation. Kinetic analysis of the templated peptide ligation revealed that the selenocystine-selenoester reaction was 10 times faster than traditional native chemical ligation at cysteine and to our knowledge is the fastest templated ligation reaction reported to date. The efficiency and operational simplicity of this technology is highlighted through the formation of hairpin molecular architectures and in a novel paper-based lateral flow assay for the rapid and sequence specific detection of oligonucleotides, including miRNA in cell lysates.
Starting Page	896
Ending Page	903
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1039/c7sc02736b
PubMed reference number	29629156
Journal	Medline
Volume Number	9
Alternate Webpage(s)	http://www.rsc.org/suppdata/c7/sc/c7sc02736b/c7sc02736b1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/c7sc02736b
Journal	Chemical science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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