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N1-Methyladenosine detection with CRISPR-Cas13a/C2c2† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc03408g
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chen, Yi Yang, Shixi Peng, Shuang Yao, Qian Wang, Fang Weng, Xiaocheng Zhou, Xiang |
| Copyright Year | 2019 |
| Abstract | Recent studies suggested that the widespread presence of N1-methyladenosine (m1A) plays a very important role in environmental stress, ribosome biogenesis and antibiotic resistance. The RNA-guided, RNA-targeting CRISPR Cas13a exhibits a "collateral effect" of promiscuous RNase activity upon target recognition with high sensitivity. Inspired by the advantage of CRISPR Cas13a, we designed a system to detect m1A induced mismatch, providing a rapid, simple and fluorescence-based m1A detection. For A-ssRNA, the Cas13a-based molecular detection platform showed a high fluorescence signal. For m1A-ssRNA, there is an about 90% decline of fluorescence. Moreover, this approach can also be used to quantify m1A in RNAs and applied for the analysis of dynamic m1A demethylation of 28S rRNA with AlkB. |
| Starting Page | 2975 |
| Ending Page | 2979 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C8SC03408G |
| PubMed reference number | 30996876 |
| Journal | Medline |
| Volume Number | 10 |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/b3/SC-010-C8SC03408G.PMC6427938.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/C8SC03408G |
| Journal | Chemical science |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
