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MicroRNA-31 predicts the presence of lymph node metastases and survival in patients with lung adenocarcinoma.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Meng, Wei Ye, Zhenqing Cui, Ri Xian Perry, James Russell Dedousi-Huebner, Vaia Huebner, Alexander Wang, Yao Li, Bin Volinia, Stefano Nakanishi, Hiromasa Kim, Taewan Suh, Sung-Suk Ayers, Leona W. Ross, Patrick Ian Croce, Carlo Maria Krishanan, Thirumoorthy Jin, Victor X. Lautenschlaeger, Timothy |
| Copyright Year | 2013 |
| Abstract | PURPOSE We conducted genome-wide miRNA-sequencing (miRNA-seq) in primary cancer tissue from patients of lung adenocarcinoma to identify markers for the presence of lymph node metastasis. EXPERIMENTAL DESIGN Markers for lymph node metastasis identified by sequencing were validated in a separate cohort using quantitative PCR. After additional validation in the The Cancer Genome Atlas (TCGA) dataset, functional characterization studies were conducted in vitro. RESULTS MiR-31 was upregulated in lung adenocarcinoma tissues from patients with lymph node metastases compared with those without lymph node metastases. We confirmed miR-31 to be upregulated in lymph node-positive patients in a separate patient cohort (P = 0.009, t test), and to be expressed at higher levels in adenocarcinoma tissue than in matched normal adjacent lung tissues (P < 0.0001, paired t test). MiR-31 was then validated as a marker for lymph node metastasis in an external validation cohort of 233 lung adenocarcinoma cases of the TCGA (P = 0.031, t test). In vitro functional assays showed that miR-31 increases cell migration, invasion, and proliferation in an ERK1/2 signaling-dependent manner. Notably, miR-31 was a significant predictor of survival in a multivariate cox regression model even when controlling for cancer staging. Exploratory in silico analysis showed that low expression of miR-31 is associated with excellent survival for T2N0 patients. CONCLUSIONS We applied miRNA-seq to study microRNomes in lung adenocarcinoma tissue samples for the first time and potentially identified a miRNA predicting the presence of lymph node metastasis and survival outcomes in patients of lung adenocarcinoma. |
| Starting Page | 181 |
| Ending Page | 190 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://clincancerres.aacrjournals.org/content/clincanres/early/2013/09/11/1078-0432.CCR-13-0320.full.pdf |
| Alternate Webpage(s) | http://clincancerres.aacrjournals.org/content/clincanres/19/19/5423.full.pdf |
| PubMed reference number | 23946296v1 |
| Alternate Webpage(s) | https://doi.org/10.1158/1078-0432.CCR-13-0320 |
| DOI | 10.1158/1078-0432.ccr-13-0320 |
| Journal | Clinical cancer research : an official journal of the American Association for Cancer Research |
| Volume Number | 19 |
| Issue Number | 19 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenocarcinoma of lung (disorder) Anatomic Node Base Sequence Biopolymer Sequencing Body tissue Diagnostic Neoplasm Staging Lymph Node Tissue Lymphadenopathy MIR31 wt Allele MicroRNAs Patients Reverse Transcriptase Polymerase Chain Reaction Silo (dataset) Structure of parenchyma of lung The Cancer Genome Atlas lymph node metastases lymph nodes t test |
| Content Type | Text |
| Resource Type | Article |
