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Downregulated miR-31 level associates with poor prognosis of gastric cancer and its restoration suppresses tumor cell malignant phenotypes by inhibiting E2F2
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Huaidong Zhang, Xiaotian Liu, Yuxin Ni, Zhaohui Lin, Yue Duan, Zipeng Shi, Yue Wang, Guoqing Li, Fan |
| Copyright Year | 2016 |
| Abstract | The miRNA microarray analysis showed that miR-31 was reduced in gastric cancer. This study further assessed miR-31 expression and role of miR-31 in gastric cancer tissues and cell lines. The data showed that miR-31 expression was down-regulated in 40 cases of gastric cancer tissues compared to the adjacent normal tissues, and low expression of miR-31 was associated with poor tumor differentiation, lymph node metastasis, advanced T stage and worse overall survival of gastric cancer patients. Ectopic expression of miR-31 reduced tumor cell viability, enhanced apoptosis, arrested tumor cells at G1 transition, and reduced tumor cell migration and invasion in SGC-7901 and MGC-803 gastric cell lines in vitro. Enforced expression of miR-31 also inhibited growth of engrafted tumors in vivo. Luciferase reporter assays and western blot revealed that E2F2 is the direct target of miR-31. E2F2 expression was upregulated in gastric cancer tissues, and inversely associated with miR-31 levels, while knockdown of E2F2 expression mimicked miR-31 anti-tumor activity in gastric cancer cells, but the ectopic expression of E2F2 rescued the miR-31-mediated inhibition in gastric cell lines. Taken together, these results demonstrated that miR-31 acts as a crucial tumor suppressive activity by inhibiting E2F2s expression. Thus, miR-31 might be a candidate therapeutic target for gastric cancer patients. |
| Starting Page | 36577 |
| Ending Page | 36589 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.oncotarget.com/index.php?journal=oncotarget&op=download&page=article&path%5B%5D=9288&path%5B%5D=28633 |
| PubMed reference number | 27174918 |
| Alternate Webpage(s) | https://doi.org/10.18632/oncotarget.9288 |
| DOI | 10.18632/oncotarget.9288 |
| Journal | Oncotarget |
| Volume Number | 7 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anatomic Node Apoptosis Body tissue Cardiac Arrest Cell Survival Cultured Cell Line Ectopic Gene Expression Forecast of outcome Inhibition MIR31 wt Allele MicroRNAs Neoplasm Metastasis Neoplasms Neoplastic Cell Patients Phenotype Soluble Guanylate Cyclase, human Stage I Carcinoma of Bladder Stomach Carcinoma Western Blotting lymph node metastases lymph nodes |
| Content Type | Text |
| Resource Type | Article |
