Loading...
Please wait, while we are loading the content...
Similar Documents
Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Murray, Rachael Z. Wylie, Fiona G. Khromykh, Tatiana Hume, David A. Stow, Jennifer L. |
| Copyright Year | 2005 |
| Abstract | A key function of activated macrophages is to secrete proinflammatory cytokines such as TNFalpha; however, the intracellular pathway and machinery responsible for cytokine trafficking and secretion is largely undefined. Here we show that individual SNARE proteins involved in vesicle docking and fusion are regulated at both gene and protein expression upon stimulation with the bacterial cell wall component lipopolysaccharide. Focusing on two intracellular SNARE proteins, Vti1b and syntaxin 6 (Stx6), we show that they are up-regulated in conjunction with increasing cytokine secretion in activated macrophages and that their levels are selectively titrated to accommodate the volume and timing of post-Golgi cytokine trafficking. In macrophages, Vti1b and syntaxin 6 are localized on intracellular membranes and are present on isolated Golgi membranes and on Golgi-derived TNFalpha vesicles budded in vitro. By immunoprecipitation, we find that Vti1b and syntaxin 6 interact to form a novel intracellular Q-SNARE complex. Functional studies using overexpression of full-length and truncated proteins show that both Vti1b and syntaxin 6 function and have rate-limiting roles in TNFalpha trafficking and secretion. This study shows how macrophages have uniquely adapted a novel Golgi-associated SNARE complex to accommodate their requirement for increased cytokine secretion. |
| Starting Page | 1 |
| Ending Page | 7 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://espace.library.uq.edu.au/data/UQ_75991/UQ75991_OA.pdf?Expires=1498926215&Key-Pair-Id=APKAJKNBJ4MJBJNC6NLQ&Signature=cbciWWWnlPoLQK4xLh4Mu~pdV9Q8X70Fu-Qc8B-vMoEms5UlyCbtZWiNjSFOG5Vcyq4Cx2sAdN2LGI8Pqa80Xz8It2rQidQwpWfStMptQ6G~-nxyZ5qhsJRnKZezEL8Tw5-0zMLwMu9xa8SuMZMcp7TCw9DP40UP0n0AwHZynUABQTRiArEadJ84KHnaN0kza0sbVA5neqd6SouoO~ssCPlVahFotT~W3zZY7zlJuQOYrAp8g7H4x5lr1kCmj2fte4UOyiqjCmKQUuuG45dGdW-RiAsAHoFf52FT4PsTADN52XneEek8v~~iUKNSRoPG7CTksRmYTp0oyS01Bjm9qg__ |
| PubMed reference number | 15640147v1 |
| Volume Number | 280 |
| Issue Number | 11 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Wall Cell secretion Docking -molecular interaction Exocytosis Golgi Apparatus Intracellular Membranes Lipopolysaccharides Neoplasms Qa-SNARE Proteins SNAP receptor SNARE complex STX6 gene Tissue membrane Tumor Necrosis Factor-alpha Vesicle (morphologic abnormality) cytokine protein expression vesicle docking |
| Content Type | Text |
| Resource Type | Article |
