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Tracking SNARE complex formation in live endocrine cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | An, Seong J. Almers, Wolfhard |
| Copyright Year | 2004 |
| Abstract | Syntaxin, synaptosome-associated protein of 25 kD (SNAP25), and vesicle-associated membrane protein/synaptobrevin are collectively called SNAP receptor (SNARE) proteins, and they catalyze neuronal exocytosis by forming a "core complex." The steps in core complex formation are unknown. Here, we monitored SNARE complex formation in vivo with the use of a fluorescent version of SNAP25. In PC12 cells, we found evidence for a syntaxin-SNAP25 complex that formed with high affinity, required only the amino-terminal SNARE motif of SNAP25, tolerated a mutation that blocks formation of other syntaxin-SNAP25 complexes, and assembled reversibly when Ca2+ entered cells during depolarization. The complex may represent a precursor to the core complex formed during a Ca2+-dependent priming step of exocytosis. |
| Starting Page | 822 |
| Ending Page | 826 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.betacell-cellbio.eu/teaching/BASIC-ITV/Tracking%20SNARE%20Complex.pdf |
| PubMed reference number | 15528447v1 |
| Volume Number | 306 |
| Issue Number | 5698 |
| Journal | Science |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Affinity Calcium ion Exocytosis Membrane Proteins Mutation Priming Exercise Protein Domain Qa-SNARE Proteins SNAP receptor Synaptosomes Vesicle (morphologic abnormality) |
| Content Type | Text |
| Resource Type | Article |
