NDLI: Sec35p, a Novel Peripheral Membrane Protein, Is Required for ER to Golgi Vesicle Docking

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Sec35p, a Novel Peripheral Membrane Protein, Is Required for ER to Golgi Vesicle Docking

Content Provider	Semantic Scholar
Author	Vanrheenen, S. M. Cao, Xiaochun Lupashin, Vladimir V.
Copyright Year	1998
Abstract	SEC35 was identified in a novel screen for temperature-sensitive mutants in the secretory pathway of the yeast Saccharomyces cerevisiae (. Genetics. 142:393-406). At the restrictive temperature, the sec35-1 strain exhibits a transport block between the ER and the Golgi apparatus and accumulates numerous vesicles. SEC35 encodes a novel cytosolic protein of 32 kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec35-1 is efficiently suppressed by YPT1, which encodes the rab-like GTPase required early in the secretory pathway, or by SLY1-20, which encodes a dominant form of the ER to Golgi target -SNARE-associated protein Sly1p. Weaker suppression is evident upon overexpression of genes encoding the vesicle-SNAREs SEC22, BET1, or YKT6. The cold-sensitive lethality that results from deleting SEC35 is suppressed by YPT1 or SLY1-20. These genetic relationships suggest that Sec35p acts upstream of, or in conjunction with, Ypt1p and Sly1p as was previously found for Uso1p. Using a cell-free assay that measures distinct steps in vesicle transport from the ER to the Golgi, we find Sec35p is required for a vesicle docking stage catalyzed by Uso1p. These genetic and biochemical results suggest Sec35p acts with Uso1p to dock ER-derived vesicles to the Golgi complex.
Starting Page	1107
Ending Page	1119
Page Count	13
File Format	PDF HTM / HTML
PubMed reference number	9606204v1
Volume Number	141
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/141/5/1107.full.pdf
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/1b/39/9802026.PMC2137178.pdf
Alternate Webpage(s)	http://jcb.rupress.org/content/141/5/1107.full.pdf
Language	English
Access Restriction	Open
Subject Keyword	Boat dock Cell secretion ER to Golgi vesicle-mediated transport Exhibits as Topic Golgi Apparatus Golgi vesicle docking SCFD1 gene SNAP receptor Secretory Pathway Tissue membrane Vesicle (morphologic abnormality) Vesicle-Trafficking Protein SEC22b YKT6 gene mutant
Content Type	Text
Resource Type	Article

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