NDLI: An intramolecular t-SNARE complex functions in vivo without the syntaxin NH2-terminal regulatory domain

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An intramolecular t-SNARE complex functions in vivo without the syntaxin NH2-terminal regulatory domain

Content Provider	Semantic Scholar
Author	Bai, Xiaoyang McNew, James A.
Copyright Year	2006
Abstract	Membrane fusion in the secretory pathway is mediated by SNAREs (located on the vesicle membrane [v-SNARE] and the target membrane [t-SNARE]). In all cases examined, t-SNARE function is provided as a three-helix bundle complex containing three approximately 70-amino acid SNARE motifs. One SNARE motif is provided by a syntaxin family member (the t-SNARE heavy chain), and the other two helices are contributed by additional t-SNARE light chains. The syntaxin family is the most conformationally dynamic group of SNAREs and appears to be the major focus of SNARE regulation. An NH2-terminal region of plasma membrane syntaxins has been assigned as a negative regulatory element in vitro. This region is absolutely required for syntaxin function in vivo. We now show that the required function of the NH2-terminal regulatory domain (NRD) of the yeast plasma membrane syntaxin, Sso1p, can be circumvented when t-SNARE complex formation is made intramolecular. Our results suggest that the NRD is required for efficient t-SNARE complex formation and does not recruit necessary scaffolding factors.
Starting Page	295
Ending Page	307
Page Count	13
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/172/2/295.full.pdf
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/0c/2f/jcb1720295.PMC2063558.pdf
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/172/2/295.full.pdf?with-ds=yes
PubMed reference number	16401725v1
Volume Number	172
Journal	The Journal of cell biology
Language	English
Access Restriction	Open
Subject Keyword	Cell secretion Membrane Fusion PersonNameUse - assigned Plasma membrane Protein Domain Qa-SNARE Proteins Regulatory Sequences, Nucleic Acid SLC3A2 gene SNAP receptor SNARE complex Secretory Pathway Tissue membrane Vesicle (morphologic abnormality) nonfunctional rRNA decay vesicle membrane
Content Type	Text
Resource Type	Article

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