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Mechanism of the inhibition of the STAT3 signaling pathway by EGCG.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Yang Ren, Xuequn Deng, Chaoyang Yang, Lu Yan, Elsa C. Y. Guo, Tao Li, Ying Wei Xu, Marvin Xuejun |
| Copyright Year | 2013 |
| Abstract | Signal transducer and activator of transcription 3 (STAT3) is an oncogene that promotes cell survival, proliferation, and motility. In the present study, we explored the mechanism involved in the inhibition by epigallocatechin-3-gallate (EGCG) of STAT3 signaling as detected by surface plasmon resonance (SPR)-binding assays and in silico docking. Stat3‑binding assay indicated that EGCG significantly interrupted Stat3 peptide binding at micromolar concentrations, and the docking experiments indicated that EGCG had a strong interaction with Arg-609, one of the key residues in the STAT3 SH2 domain that contributes greatly to Stat3 and phosphorylated peptide binding. Following treatment of the hepatocellular carcinoma cell lines BEL-7402 and QGY-7703 with EGCG, in vitro, EGCG significantly suppressed cell proliferation as detected by MTT assay, induced apoptosis as detected by flow cytometry, dramatically lowered the expression levels of phosphorylated Stat3 proteins (p-Stat3) as determined by immunoblot detection, and inhibited the expression of multiple genes including Bcl-xL, c-Myc, VEGF and cyclin D1 as demonstrated by RT-PCR analysis. In conclusion, our research data indicate that the anticancer function of green tea results from the inhibition of the STAT3 signaling pathway by EGCG. |
| Starting Page | 266006 |
| Ending Page | 266006 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/or/30/6/2691/download |
| PubMed reference number | 24065300v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/or.2013.2743 |
| DOI | 10.3892/or.2013.2743 |
| Journal | Oncology reports |
| Volume Number | 30 |
| Issue Number | 6 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis BCL-XL Protein Boat dock Cell Proliferation Cell Survival Docking -molecular interaction Flow Cytometry Immunoblotting Liver carcinoma Micromole/liter Oncogenes Plant Extracts SH2 Domain Signal Transduction Pathways Stat3 protein Surface Plasmon Resonance Transducers epigallocatechin gallate monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
