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Induction of apoptosis in hormone-resistant human prostate cancer PC3 cells by inactivated Sendai virus.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gao, Hui Gong, Xiao Cheng Chen, Ze Dong Xu, Xiao Shuang Zhang, Quan Xu, Xiang Ming |
| Copyright Year | 2014 |
| Abstract | OBJECTIVE Inactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, the molecular mechanism of apoptosis induction in cancer cells mediated by HVJ-E has not been fully elucidated. This paper aims to investigate the underlying mechanism of apoptosis induction by HVJ-E in prostate cancer cells (PC3). METHODS PC3 cells were treated with HVJ-E at various MOI, and then interferon-β (IFN-β) production, and the cell viability and apoptosis were detected by ELISA, MTT-based assay and flow cytometry, respectively. Next, the roles of Jak-Stat, MAPK and Akt pathways played in HVJ-E-induced apoptosis in PC3 cells were analyzed by immunoblot assay. To further evaluate the cytotoxic effect of HVJ-E on PC3 cells, HVJ-E was intratumorally injected into prostate cancers on BALB/c-nude mice, and the tumor volume was monitored for 36 days. RESULTS HVJ-E induced IFN-β production and activated Jak-Stat signaling pathway, which resulted in the activation of caspase-8, caspase-3, and PARP in PC3 prostate cancer cells post HVJ-E treatment. Furthermore, we observed for the first time that p38 and Jnk MAPKs in PC3 cells contributed to HVJ-E-induced apoptosis. In addition, intratumoral HVJ-E treatment displayed a direct inhibitory effect in an in vivo BALB/c nude mouse prostate cancer model. CONCLUSION Our findings have provided novel insights into the underlying mechanisms by which HVJ-E induces apoptosis in tumor cells. |
| Starting Page | 3260 |
| Ending Page | 3266 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.besjournal.com/Articles/Archive/2014/No7/201407/P020140729369401300174.pdf |
| PubMed reference number | 25073909v1 |
| Alternate Webpage(s) | https://doi.org/10.3967/bes2014.082 |
| DOI | 10.3967/bes2014.082 |
| Journal | Biomedical and environmental sciences : BES |
| Volume Number | 27 |
| Issue Number | 7 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Survival Flow Cytometry HIV Immunoblotting Mice, Nude Murine sarcoma viruses PARP1 protein, human Positive Regulation of Apoptosis Prostatic Neoplasms Signal Transduction Pathways Tumor Volume Virion cancer cell caspase caspase-3 caspase-8 monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
