NDLI: Epigallocatechin gallate attenuated the activation of rat cardiac fibroblasts induced by angiotensin II via regulating β-arrestin1.

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Epigallocatechin gallate attenuated the activation of rat cardiac fibroblasts induced by angiotensin II via regulating β-arrestin1.

Content Provider	Semantic Scholar
Author	Han, Yong-Sheng Lan, Lei Chu, Jun Uk Kang, Wei-qiang Ge, Zhi-ming
Copyright Year	2013
Abstract	BACKGROUND/AIMS Angiotensin II (AngII) activated cardiac fibroblasts (CFs) predominantly through AngII subtype 1a receptor (AT1aR). This study was carried out to explore the potential inhibitory effects and mechanisms of epigallocatechin gallate (EGCG) on AngII induced rat CFs. METHODS Viability, proliferation and collagen production of CFs were measured by MTT assay, [3H]-thymidine and [3H]-proline incorporation respectively. β-arrestin1 (βarr1), AT1aR and AT1bR mRNA levels were determined by quantitative PCR. AT1R, Gq, βarr 1/2, phosphorylated kinase C (p-PKC)-delta expressions were detected by western blotting. We blocked βarr1 expression using βarr1 small interfering RNA (siRNA). RESULTS EGCG inhibited the activation of CFs induced by AngII. βarr1 mRNA level revealed a positive correlation with the viability of CFs. SiRNA targeting βarr1 blocked the activation of CFs. In vitro, AngII increased βarr1 mRNA, total and membrane βarr1 protein expressions, but reduced AT1aR mRNA, global and membrane AT1R, total Gq and cytoplasmic p-PKC-delta levels. Administration of EGCG restored the above abnormalities, whereas Gq levels were not affected. CONCLUSION Our findings showed that βarr1 is essential for AngII-mediated activation of CFs. EGCG attenuated CFs activation induced by AngII via regulating βarr1 and thus, modulating AT1aR mediated signaling.
Starting Page	479
Ending Page	481
Page Count	3
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.karger.com/Article/Pdf/343371
PubMed reference number	23485661v1
Alternate Webpage(s)	https://doi.org/10.1159/000343371
DOI	10.1159/000343371
Journal	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Volume Number	31
Issue Number	2-3
Language	English
Access Restriction	Open
Subject Keyword	Angiotensin II Angiotensins Congenital Abnormality Myocytes, Cardiac Non-Small Cell Lung Carcinoma RNA RNA, Small Interfering Tissue membrane Western Blotting epigallocatechin gallate monooxyethylene trimethylolpropane tristearate
Content Type	Text
Resource Type	Article

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