Loading...
Please wait, while we are loading the content...
Similar Documents
Sulforaphane inhibits the proliferation of the BIU87 bladder cancer cell line via IGFBP-3 elevation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dang, Ya-Mei Huang, Gang Chen, Yi-Rong Dang, Zhong-Feng Chen, Cheng Liu, Feng-Lei Guo, Ying-Fang Xie, Xiao-Dong |
| Copyright Year | 2014 |
| Abstract | AIM To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involving IGFBP-3. METHODS Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry. Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3 and NF-κB at both mRNA and protein levels. RESULTS Sulforaphane (80 μM) treatment could inhibit cell proliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized by IGFBP-3 silencing. Furthermore, sulforaphane (80 μM) could down-regulate NF-κB expression while elevating that of IGFBP-3. CONCLUSIONS Sulforaphane could suppress the proliferation of BIU87 cells via enhancing IGFBP-3 expression, which negatively regulating the NF-κB signaling pathway. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ocean.kisti.re.kr/downfile/volume/apocp/POCPA9/2014/v15n4/POCPA9_2014_v15n4_1517.pdf |
| PubMed reference number | 24641360v1 |
| Volume Number | 15 |
| Issue Number | 4 |
| Journal | Asian Pacific journal of cancer prevention : APJCP |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Cell Cycle Arrest Cell Proliferation Eighty Flow Cytometry IGFBP3 protein, human Mitosis Neoplasms Polymerase Chain Reaction Signal Transduction Pathways Western Blotting cancer cell monooxyethylene trimethylolpropane tristearate sulforafan |
| Content Type | Text |
| Resource Type | Article |
