Loading...
Please wait, while we are loading the content...
Similar Documents
RecF and RecR Play Critical Roles in the Homologous Recombination and Single-Strand Annealing Pathways of Mycobacteria.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gupta, Richa Shuman, Stewart Glickman, Michael S. |
| Copyright Year | 2015 |
| Abstract | Mycobacteria encode three DNA double-strand break repair pathways: (i) RecA-dependent homologous recombination (HR), (ii) Ku-dependent nonhomologous end joining (NHEJ), and (iii) RecBCD-dependent single-strand annealing (SSA). Mycobacterial HR has two presynaptic pathway options that rely on the helicase-nuclease AdnAB and the strand annealing protein RecO, respectively. Ablation of adnAB or recO individually causes partial impairment of HR, but loss of adnAB and recO in combination abolishes HR. RecO, which can accelerate annealing of single-stranded DNA in vitro, also participates in the SSA pathway. The functions of RecF and RecR, which, in other model bacteria, function in concert with RecO as mediators of RecA loading, have not been examined in mycobacteria. Here, we present a genetic analysis of recF and recR in mycobacterial recombination. We find that RecF, like RecO, participates in the AdnAB-independent arm of the HR pathway and in SSA. In contrast, RecR is required for all HR in mycobacteria and for SSA. The essentiality of RecR as an agent of HR is yet another distinctive feature of mycobacterial DNA repair.IMPORTANCE This study clarifies the molecular requirements for homologous recombination in mycobacteria. Specifically, we demonstrate that RecF and RecR play important roles in both the RecA-dependent homologous recombination and RecA-independent single-strand annealing pathways. Coupled with our previous findings (R. Gupta, M. Ryzhikov, O. Koroleva, M. Unciuleac, S. Shuman, S. Korolev, and M. S. Glickman, Nucleic Acids Res 41:2284-2295, 2013, http://dx.doi.org/10.1093/nar/gks1298), these results revise our view of mycobacterial recombination and place the RecFOR system in a central position in homology-dependent DNA repair. |
| Starting Page | 1335 |
| Ending Page | 1345 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=EBBA0F293F8E20072BDF1B6D3BED52C1?doi=10.1.1.1032.5657&rep=rep1&type=pdf |
| Alternate Webpage(s) | http://jb.asm.org/content/197/19/3121.full.pdf |
| PubMed reference number | 26195593v1 |
| Alternate Webpage(s) | https://doi.org/10.1128/JB.00290-15 |
| DOI | 10.1128/jb.00290-15 |
| Journal | Journal of bacteriology |
| Volume Number | 197 |
| Issue Number | 19 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus DNA Breaks, Single-Stranded DNA Repair Double Strand Break Repair Encode (action) Genus Mycobacterium Homologous Gene In Situ Nick-End Labeling Non-Homologous DNA End-Joining Nucleic Acid Strand RAD51 gene Recombination, Genetic double-strand break repair via homologous recombination nuclease |
| Content Type | Text |
| Resource Type | Article |
