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Growing dictyate oocytes, but not early preimplantation embryos, of the mouse display high levels of DNA homologous recombination by single-strand annealing and lack DNA nonhomologous end joining.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fiorenza, Maria Teresa Bevilacqua, Antonio Bevilacqua, Stanislao Mangia, Federica |
| Copyright Year | 2001 |
| Abstract | We have investigated the ability of growing dictyate oocytes and early preimplantation embryos of the mouse to process extrachromosomal DNA molecules with free ends by intranuclearly microinjecting DNA fragments containing a region of homology of various extent at either the 5' or 3' terminus. Homologous recombination of these fragments by single-strand annealing (SSA), but not other DNA recombination/joining mechanisms, resulted in the formation of a full-length hsp-lacZ-pA fusion gene that was transcriptionally activated by heat shock in growing oocytes and spontaneously at the early two-cell stage in the embryos, making it possible to quantitatively evaluate SSA activities of these cells by the beta-galactosidase produced. SSA activities of oocytes and embryos were similar in their general properties and in the activity levels observed with saturating amounts of DNA. However, embryo SSA was almost one order of magnitude less effective than that of oocytes. Oocyte and embryo 5' --> 3' exonuclease (a key function of the SSA pathway) and DNA nonhomologous end joining (NHEJ) activities were also investigated using an asymmetric PCR assay. Results showed that NHEJ is lacking in oocytes and is very prominent in the embryos, where it competes with SSA for the injected DNA. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/82434303.pdf |
| PubMed reference number | 11319870v1 |
| Volume Number | 233 |
| Issue Number | 1 |
| Journal | Developmental biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | DNA Breaks, Single-Stranded Embryo Exonuclease Galactosidase Heat-Shock Response Homologous Gene In Situ Nick-End Labeling Non-Homologous DNA End-Joining Oocytes Preimplantation Diagnosis Recombination, Genetic Saturated Shock beta-Galactosidase double-strand break repair via homologous recombination |
| Content Type | Text |
| Resource Type | Article |
